Characterization of Human Pericyte Phenotype by Immunohistochemistry

Pericytes are found in all vascularized organs and are defined anatomically as perivascular cells that closely surround endothelial cells in capillaries and microvessels and are embedded within the same basement membrane. They have been shown to have diverse physiological and pathological functions...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2235; p. 37
Main Authors West, Christopher C, Khan, Nusrat S, Crisan, Mihaela
Format Journal Article
LanguageEnglish
Published United States 2021
Subjects
Capillaries - cytology
Cell Differentiation - physiology
Cells, Cultured
Coculture Techniques
Endothelial Cells - cytology
Humans
Immunohistochemistry - methods
Microvessels - cytology
Pericytes - cytology
Pericytes - metabolism
Pericytes - transplantation
Phenotype
Immunohistochemistry
Pericytes
Mesenchymal stem/stromal cells
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Summary:Pericytes are found in all vascularized organs and are defined anatomically as perivascular cells that closely surround endothelial cells in capillaries and microvessels and are embedded within the same basement membrane. They have been shown to have diverse physiological and pathological functions including regulation of blood pressure, and tissue regeneration and scarring. Fundamental to understanding the role these cells play in these diverse processes is the ability to accurately identify and localize them in vivo. To do this, we have developed multicolor immunohistochemistry protocols described in this chapter.
ISSN:1940-6029
DOI:10.1007/978-1-0716-1056-5_4