The potential of Clostridium autoethanogenum, a new single cell protein, in substituting fish meal in the diet of largemouth bass (Micropterus salmoides): Growth, feed utilization and intestinal histology

• Published in: Aquaculture and fisheries Vol. 8; no. 1; pp. 67 - 75
• Main Authors: Yang, Pinxian, Li, Xiaoqin, Song, Bowen, He, Ming, Wu, Chaoyi, Leng, Xiangjun
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.01.2023
• Subjects: Clostridium autoethanogenum protein; Feed utilization; Growth performance; Intestinal histology; Largemouth bass; Intestinal histology; Largemouth bass; Clostridium autoethanogenum protein; Growth performance; Feed utilization
• ISSN: 2468-550X; 2468-550X
• DOI: 10.1016/j.aaf.2021.03.003

This study investigated the effects of replacing fishmeal with Clostridium autoethanogenum protein (CAP) on growth, feed utilization and intestinal histology of largemouth bass (Micropterus salmoides). The control diet contained 350 g/kg fishmeal, and then dietary fishmeal was decreased to 300 g/kg, 250 g/kg, 200 g/kg, 150 g/kg and 100 g/kg by CAP inclusion, respectively (FM-30, FM-25, FM-20, FM-15, FM-10). The largemouth bass (110.0 ± 1.0 g) were fed one of the six diets for 8 weeks. The groups of FM-30, FM-25 and FM-20 showed the similar weight gain (WG) and feed conversion ratio (FCR) to the control (P > 0.05), while the WG of FM-10 group was decreased by 15.9%, and FCR increased by 0.12 when compared to the control (P < 0.05). FM-15 and FM-10 groups had lower protein retention, lower villus height in intestine and higher serum malondialdehyde content than the control (P < 0.05). The protease activity in FM-20, FM-15 and FM-10 groups was lower than that in the control (P < 0.05), but no difference was observed in crude protein digestibility among groups (P > 0.05). In conclusion, CAP could replace 150 g/kg fishmeal in diet (350 g/kg fishmeal) without adverse effects on growth, feed utilization and intestinal histology of largemouth bass.