Increased production of alkaline polygalacturonate lyase in the recombinant WB43CB by optimizing the medium

Alkaline polygalacturonate lyase (PGL), which was the central enzyme of green environmental enzyme treatment technology, has been widely used in textile, papermaking and beverage production. In the previous work, Bacillus subtilis was used as the expression host, and the PGL gene derived from B. sub...

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Bibliographic Details
Published inE3S Web of Conferences Vol. 251; p. 2053
Main Authors Zhang, Junjiao, Zhang, Junli
Format Journal Article Conference Proceeding
LanguageEnglish
Published Les Ulis EDP Sciences 01.01.2021
Subjects
Carbon sources
Enzymatic activity
Enzyme activity
Enzymes
Fermentation
Industrial production
Inoculum
Papermaking
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Summary:Alkaline polygalacturonate lyase (PGL), which was the central enzyme of green environmental enzyme treatment technology, has been widely used in textile, papermaking and beverage production. In the previous work, Bacillus subtilis was used as the expression host, and the PGL gene derived from B. subtilis WSHB04-02 was successfully expressed in the engineered strain WB43CB by regulating of molecular elements such as signal peptide, promoter and SD sequence. In this study, the fermentation medium was optimized from four aspects of carbon source, nitrogen source, liquid volume and inoculum volume, and the extracellular PGL enzyme activity in WB43CB increased from 264.5 U·mL -1 to 461.96 U·mL -1 , which laid a solid foundation for the industrial production of PGL.
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/202125102053