Oat Doubled Haploids Following Maize Pollination

Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. These...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1536; p. 23
Main Authors Davies, Philip A, Sidhu, Parminder K
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Avena - genetics
Avena - growth & development
Breeding
Crosses, Genetic
Haploidy
Phenotype
Pollination
Seeds - genetics
Zea mays - genetics
Avena sativa
Oat
Zea mays
Breeding
Haploid
Maize
Doubled haploid
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Summary:Doubled haploids (DHs) are an important tool for the accelerated production of new crop varieties. In oat, DHs were first produced by pollinating oat florets with maize pollen. The resultant embryos spontaneously eliminate the maize chromosomes leaving a haploid complement of oat chromosomes. These embryos can be cultured in vitro using the "embryo rescue" technique to produce haploid plants whose chromosome number can be doubled with colchicine to produce homozygous DH oat plants.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6682-0_2