Ex Vivo Hematopoietic Progenitor Cell Expansion

The ability to culture and expand hematopoietic progenitor cells ex vivo has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This rev...

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Published inImmunoMethods (San Diego, Calif.) Vol. 5; no. 3; pp. 217 - 225
Main Authors Haylock, D.N., Makino, S., Dowse, T.L., Trimboli, S., Niutta, S., To, L.B., Juttner, C.A., Simmons, P.J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.1994
Subjects
Antigens, CD - analysis
Antigens, CD34
Cell Separation
Cells, Cultured
Culture Techniques - methods
Hematopoiesis - physiology
Humans
Stem Cells - cytology
Stem Cells - immunology
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Summary:The ability to culture and expand hematopoietic progenitor cells ex vivo has major implications for both bone marrow and stem cell support following marrow ablative or subablative high-dose therapy and for improving the efficiency of retroviral transfection in gene marking and gene therapy. This review focuses on methods for the generation of myeloid progenitor and post-progenitor cells from peripheral blood stem cell collections, with particular emphasis on the characterization of these cells and practical issues associated with their expansion.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
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ObjectType-Review-1
ISSN:1058-6687
1557-766X
DOI:10.1006/immu.1994.1059