Comparison of a monoclonal antibody fluorescent polarization immunoassay with monoclonal antibody radioimmunoassay for cyclosporin determination in whole blood

We have compared the whole blood concentrations of parent cyclosporin A (CsA) using monoclonal fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA) as well as polyclonal FPIA in kidney, heart, and bone marrow transplant patients (n = 89). A good correlation was found between monoc...

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Bibliographic Details
Published inTherapeutic drug monitoring Vol. 14; no. 1; p. 78
Main Authors Alvarez, J S, Sacristan, J A, Alsar, M J
Format Journal Article
LanguageEnglish
Published United States 01.02.1992
Subjects
Antibodies, Monoclonal
Bone Marrow Transplantation - immunology
Cyclosporine - blood
Fluorescence Polarization Immunoassay
Heart Transplantation - immunology
Humans
Kidney Transplantation - immunology
Radioimmunoassay
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Summary:We have compared the whole blood concentrations of parent cyclosporin A (CsA) using monoclonal fluorescence polarization immunoassay (FPIA) and radioimmunoassay (RIA) as well as polyclonal FPIA in kidney, heart, and bone marrow transplant patients (n = 89). A good correlation was found between monoclonal FPIA and monoclonal RIA (r = 0.96) and a slightly worse one between polyclonal and monoclonal FPIA (r = 0.90). The interassay coefficient of variation was satisfactory for all the methods-less than 5% for monoclonal FPIA. The monoclonal FPIA assay with Abbott TDx appears to provide rapid, precise, and accurate measurement of parent CsA. It is therefore useful for therapeutic monitoring of CsA in whole blood in kidney, heart, and bone marrow transplant patients.
ISSN:0163-4356
DOI:10.1097/00007691-199202000-00013