Purification, Structural Elucidation, and Anticancerous Properties of a Novel Flavonoid from Flowers of Leucas indica

Previously, we showed that the crude methanol extracts of Leucas indica flowers exhibited antioxidant properties and in the current study, crude methanol flower extracts of L. indica showed anticancerous properties as evidenced cytotoxicity (MTT assay test) against the selected cancerous cell lines...

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Bibliographic Details
Published inProcesses Vol. 10; no. 11; p. 2341
Main Authors Sowjanya, Muthyam, Venkata Sandeep, Bhagavathula, Venkaiah, Kaduru, Dirisala, Vijaya R., Reddy, Malapati Hanuma, Pradeepkiran, Jangampalli Adi, Sainath, Sri Bhashyam
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.11.2022
Subjects
Antimicrobial agents
Antioxidants
Bioactive compounds
Biocompatibility
Biological activity
Breast cancer
Cancer therapies
Cell culture
Cell lines
Chromatography
Column chromatography
Cytotoxicity
Drugs
Flavonoids
Flowers
Flowers & plants
Fractionation
Leucas
Mass spectroscopy
Medical research
Methanol
Natural products
NMR
Nuclear magnetic resonance
Silica gel
Tetrahydro-2H-pyran
Toxicity testing
Typhoid
St Louis Missouri
United States > US
India
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Summary:Previously, we showed that the crude methanol extracts of Leucas indica flowers exhibited antioxidant properties and in the current study, crude methanol flower extracts of L. indica showed anticancerous properties as evidenced cytotoxicity (MTT assay test) against the selected cancerous cell lines HeLa, HCT116, HL-60, and MCF-7. Therefore, further analysis was performed to isolate and purify the bioactive compound using activity-guided repeated fractionation of the methanol extract by silica gel column chromatography. After collection of different fractions, all the fractions were subjected to TLC analysis and the fractions which yielded the same compounds on TLC were further analyzed for physicochemical and spectroscopic analyses, e.g., UV, IR, 1H NMR, 13C NMR, COSY, HSQC, and mass spectroscopy. The bioactive compound isolated was elucidated as 6-hydroxy-3-(4-hydroxyphenyl)-7-(3,4,5-trihydroxy-6-)(hydroxymethyl)tetrahydro-2H-pyran-2yl)-4H-chromen-4-one. Based on the antioxidant and anticancerous properties, L. indica might be a promising source of useful natural products and the newly bioactive compound might offer opportunities to develop new anticancerous drugs.
ISSN:2227-9717
2227-9717
DOI:10.3390/pr10112341