Dietary Effects of Probiotic Bacteria, Bacillus amyloliquefaciens AV5 on Growth, Serum and Mucus Immune Response, Metabolomics, and Lipid Metabolism in Nile Tilapia ( Oreochromis niloticus )

• Published in: Aquaculture nutrition Vol. 2024; no. 1
• Main Authors: Shija, Vicent Michael, Zakaria, Glory Emanuel, Amoah, Kwaku, Yi, Li, Huang, Junwei, Masanja, Fortunatus, Yong, Zhong, Cai, Jia
• Format: Journal Article
• Language: English
• Published: Oxford Hindawi Limited 01.01.2024; Hindawi-Wiley
• Subjects: Aquaculture; Bacillus amyloliquefaciens; Bacteria; Diet; Disease; Feed conversion; Feeds; Fish; Food conversion; Growth rate; Immune response; Infections; Laboratory animals; Lipids; Marine fishes; Metabolism; Metabolites; Mucus; Oreochromis niloticus; Phosphatase; Probiotics; Serum; Survival; Tilapia; Water quality; Weight; China
• ISSN: 1353-5773; 1365-2095
• DOI: 10.1155/2024/4253969

In the present study, we investigated the effect of dietary supplementation with the probiotic Bacillus amyloliquefaciens AV5 (OR647358) on the growth, serum and mucus immune responses, metabolomics, and lipid metabolism of Oreochromis niloticus . Fishes (27.2 ± 1.7 g and 9.0 ± 1.2 cm) were fed three distinct meals: a commercial diet (control‐GC) and two treatment diets supplemented with probiotics at 10 6 (G1) and 10 8 cfu/g (G2), respectively, for 30 days. In the G2 group, the final weight, specific growth rate, weight gain rate, survival rate, and feed conversion ratio of the fish were significantly improved ( p < 0.05). Lysozyme, myeloperoxidase, and alkaline phosphatase activities in the mucus of fish were significantly higher ( p < 0.05) in the G1 and G2 groups. The serum total protein, superoxide dismutase, glutathione peroxidase, reactive oxygen species, and reactive nitrogen species levels were noticeably higher ( p < 0.05) in fish fed G1 and G2. In addition, in the G1 and G2 groups, higher levels of enzymes involved in lipid metabolism, such as pyruvate kinase, 2‐hydroxyethyl‐ThPP, and dihydrolipoamide dehydrogenase, were increased. Distal gastrointestinal metabolites, such as glycerophospholipids and histidine, were observed. These findings strongly indicate that incorporating B. amyloliquefaciens AV5 at 10 8 cfu/g into commercial feeds positively influences fish growth, immunity, and lipid metabolism.