Tyrosine kinase phosphorylation of GABAA receptor subunits following chronic ethanol exposure of cultured cortical neurons of mice

• Published in: Brain research Vol. 1086; no. 1; pp. 35 - 41
• Main Authors: RAUINDRAN, C. R. Marutha, TICKU, Maharaj K
• Format: Journal Article
• Language: English
• Published: London Elsevier 01.05.2006; Amsterdam; New York, NY
• Subjects: Addictive behaviors; Adult and adolescent clinical studies; Alcoholism; Alcoholism and acute alcohol poisoning; Animals; Biological and medical sciences; Blotting, Western - methods; Central Nervous System Depressants - pharmacology; Cerebral Cortex - cytology; Embryo, Mammalian; Ethanol - pharmacology; Female; Gene Expression Regulation - drug effects; Immunoprecipitation - methods; Medical sciences; Mice; Neurons - drug effects; Phosphorylation - drug effects; Pregnancy; Protein Subunits - metabolism; Protein-Tyrosine Kinases - metabolism; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Receptors, GABA-A - metabolism; Time Factors; Toxicology; Cerebral cortex; Phosphorylation; Ethanol; Enzyme; Transferases; Alcoholism; Rodentia; Central nervous system; Exposure; Subunit; In vitro; Encephalon; Vertebrata; Tyrosine kinase phosphorylation;Chronic ethanol;Cultured cortical neuron;GABAA receptor subunit; Mammalia; Mouse; Animal; Protein-tyrosine kinase; Gabaergic receptor A
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2006.02.106

Previous studies from our laboratory revealed that acute ethanol exposure inhibits phosphorylation of mitogen-activated protein (MAP) kinase and extracellular signal-regulated kinases (ERK) in mice. In the present study, we have further investigated effect of chronic administration of ethanol on tyrosine kinase phosphorylation of GABA(A) receptor subunits in the mouse cultured cortical neurons. We observed that there was an up-regulation in tyrosine kinase phosphorylation of the GABA(A) receptor beta(2) and gamma(2) subunits following chronic ethanol exposure, whereas there was no effect on alpha(1) subunit of the GABA(A) receptor in the cultured cortical neurons of mice as determined by Western blotting. These results suggest a potential role for tyrosine kinase phosphorylation of some of the GABA(A) receptor subunits in chronic ethanol-induced tolerance and dependence.