Fast Measurement of Lipid Content of Oleaginous Yeast Trichosporon dermatis Cultured in Lignocellulosic Hydrolysates Using Fluorescent Method

• Published in: Journal of the Chemical Society of Pakistan Vol. 43; no. 1; p. 80
• Main Authors: Cheng Zhao, Cheng Zhao, Qing Song Yao, Qing Song Yao, Can Wang, Can Wang, Mu Tan Luo, Mu Tan Luo, Chao Huang, Chao Huang, Xue Fang Chen, Xue Fang Chen, Lan Lan Tian, Lan Lan Tian, Qian Lin Huang, Qian Lin Huang, Lian Xiong, Lian Xiong, Hai Long Li, Hai Long Li, Xin De Chen, Xin De Chen
• Format: Journal Article
• Language: English
• Published: Knowledge Bylanes 28.02.2021
• Subjects: Analysis; Composition; Fluorescence; Lipids; Methods; Physiological aspects; Yeast fungi; China
• ISSN: 0253-5106
• DOI: 10.52568/000010

To avoid complex procedures in measurement of lipid content of oleaginous yeast especially for that can accumulate microbial lipid in lignocellulosic hydrolysates, fluorescent method using Nile Red as fluorescent dye was applied to measure lipid content of oleaginous yeast Trichosporon dermatis. The fluorescent method was built by fitting of lipid content identified by both conventional gravimetric method and fluorescence intensity of oleaginous yeast. Within the range of lipid content measured, the fitting curves showed linear relationship with good correlation coefficient (R2=0.95), showing this method is suitable for measuring lipid content of T. dermatis in the simulated medium. To evaluate the applicability of this method for lipid fermentation using lignocellulosic acid hydrolysates as substrate, T. dermatis was cultured in corncob acid hydrolysate and rice straw acid hydrolysate and then its lipid content measured by both fluorescent method and gravimetric method were compared. The results showed that the lipid content measured by these two methods were close, therefore, this method was promising for the application in lipid fermentation in lignocellulosic acid hydrolysates.