Two new thermostable type II restriction endonucleases from Thermus aquaticus: TatI and TauI, which recognize the novel nucleotide sequences 5′-W↓GTACW-3′ and 5′-GCSG↓C-3′ respectively

• Published in: FEMS microbiology letters Vol. 204; no. 2; pp. 253 - 257
• Main Authors: Vitkute, J, Maneliene, Z, Janulaitis, A
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier B.V 13.11.2001; Blackwell
• Subjects: Bacteriology; Base Sequence; Biological and medical sciences; Deoxyribonucleases, Type II Site-Specific - metabolism; DNA - chemistry; DNA - metabolism; Enzyme Stability; Fundamental and applied biological sciences. Psychology; Genetics; Hot Temperature; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Restriction endonuclease; Substrate Specificity; Thermostable; Thermus; Thermus - enzymology; Thermostable; Thermus; Restriction endonuclease; Nucleotide sequence; Thermus aquaticus; Enzyme; Thermophily; Thermal stability; Screening; Type; Nucleotide; Bacteria; Isolate; Isolation; Recognition
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(01)00410-4

One hundred and forty isolates of thermophilic bacteria from the genus Thermus were screened for the presence of restriction endonuclease activity. Thermostable isoschizomers of restriction endonucleases, such as AceIII, BbvI, BglI, BsePI, FnuDII, HgiAI, MaeII, MboI, MseI, PvuII, StuI, TaqI, Tsp4CI, TspEI, XhoI and XmaIII, were isolated. Two restriction enzymes, TatI and TauI, recognizing novel degenerate sequences 5′-W↓GTACW-3′ and 5′-GCSG↓C-3′ respectively were partially purified and the recognition and cleavage sites were determined.