Low temperatures enhance the absorption and translocation of 14C-glyphosate in glyphosate-resistant Conyza sumatrensis

• Published in: Journal of plant physiology Vol. 240; p. 153009
• Main Authors: Palma-Bautista, Candelario, Alcántara-de la Cruz, Ricardo, Rojano-Delgado, Antonia M., Dellaferrera, Ignacio, Domínguez-Martínez, Pablo Alfredo, De Prado, Rafael
• Format: Journal Article
• Language: English
• Published: Stuttgart Elsevier GmbH 01.09.2019; Elsevier Science Ltd
• Subjects: 5-enolpyruvyishikimate 3-phosphate synthase; Absorption; Accumulation; Biotypes; Conyza sumatrensis; Enzymatic activity; Glyphosate; Glyphosate translocation; Herbicides; Herbivores; High temperature; Leaves; Low temperature; Low temperature resistance; Roots; Shikimic acid; Sumatran fleabane; Temperature; Translocation; Vacuolar sequestration; Glyphosate translocation; Sumatran fleabane; Vacuolar sequestration; 5-enolpyruvyishikimate 3-phosphate synthase
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2019.153009

Influence of low temperatures on the glyphosate efficacy was studied in glyphosate-resistant (R) and -susceptible (S) Conyza sumatrensis biotypes. For this purpose, the physiological and enzymatic aspects involved were characterized under two growing temperature regimes [high (30/20 °C) and low 15/5 °C temperatures day/night]. The R biotype was 5.5 times more resistant than the S biotype at high temperatures; however, this R-to-S ratio decreased to 1.6 at low temperatures. At 96 h after treatment (HAT), the shikimic acid accumulation was higher in the S biotype in both temperature regimes (4.6 and 1.9 more shikimic acid at high and low temperatures, respectively), but the accumulation of the R biotype increased 2.6 times at low temperatures compared to high ones. From 24 to 96 HAT, the 14C-glyphosate absorption ranged from 28 to 65% (percentage reached from 48 HAT) at low temperatures, and from 20 to 50% at high temperatures (gradual increase), but there were no differences between C. sumatrensis biotypes within each temperature regime. At high temperatures, the 14C-glyphosate translocation was different between biotypes, where the R one retained at least 10% more herbicide in the treated leaves than the S biotype at 96 HAT. So, the S biotype translocated 40% of 14C-glyphosate absorbed to roots, and the R biotype translocated only 28% of herbicide at the same period. At low temperatures, there were no differences between biotypes, and at 96 HAT, the 14C-glyphosate found in treated leaves was ˜47% and up to ˜42% reached the roots, i.e., the resistance mechanism was suppressed. The basal and enzymatic activities of the 5-enolpyruvyishikimate 3-phosphate synthase were different between temperature regimes, but there was no differences between biotypes within each temperature regime, showing that target-site resistance mechanisms did not contribute in the glyphosate resistance of the R biotype. Low temperatures enhanced the absorption and translocation of glyphosate by suppressing the resistance mechanisms improving its efficacy on resistant plants. This is the first characterization about the role of temperatures in the glyphosate efficacy on C. sumatrensis.