Peanut agglutinin. II. Characterization of the Thy-1, Tla and Ig phenotype of peanut agglutinin-positive cells in adult, embryonic and nude mice using double immunofluorescence

• Published in: European journal of immunology Vol. 9; no. 2; p. 139
• Main Authors: Roelants, G E, London, J, Mayor-Withey, K S, Serrano, B
• Format: Journal Article
• Language: English
• Published: Germany 01.02.1979
• Subjects: Animals; Antigens, Surface - analysis; Classification; Isoantigens - analysis; Lectins - immunology; Liver - immunology; Lymphocytes - immunology; Lymphoid Tissue - immunology; Mice; Mice, Inbred AKR - immunology; Mice, Inbred NZB - immunology; Mice, Nude - immunology; Receptors, Antigen, B-Cell - analysis; T-Lymphocytes - immunology
• ISSN: 0014-2980
• DOI: 10.1002/eji.1830090209

The Thy-1, Tla and Ig phenotype of peanut agglutinin (PNA)-binding cells was characterized in various strains of mice. In the thymus, PNA was found to bind principally but not exclusively to the Thy-1+ Tla + Ig-- steroid-sensitive cortical thymocytes. Thy1 + Tla -- Ig -- steroid-resistant cells are not labeled with PNA. In other lymphoid organs, PNA bound to a minority of T or null cells but generally not to B cells. During ontogeny, PNA + and PNA -- T lineage cells appear simultaneously in the liver at day 10 of gestation, in the thymus at day 11 and in the spleen at day 18. No evidence was found for a maturation from PNA + to PNA -- cells. Prethymocytes present in nude mice were also divided into a PNA + and PNA -- population.