Immunostimulatory characteristics of a novel adjuvant on the basis of cucumarioside A₂-2 and monogalactosyldiacylgycerol

A novel antigen carrier has been formulated on the basis of a cucumarioside-A₂-2 triterpene glycoside (CD) complex with cholesterol and monogalactosyldiacylglycerol from Ahnfeltia tobuchiensis (MGDGAt) and Ulva fenestrate (MGDGUf). Morphological and immunostimulative characteristics of the carrier w...

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Published inApplied biochemistry and microbiology Vol. 44; no. 6; pp. 629 - 634
Main Authors Li, I. A, Popov, A. M, Tsybul'skii, A. V, Sanina, N. M, Kostetskii, E. Ya, Novikova, O. D, Portnyagina, O. Yu, Mazeika, A. V
Format Journal Article
LanguageEnglish
Published Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.11.2008
SP MAIK Nauka/Interperiodica
Springer Nature B.V
Subjects
Algae
Antigens
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cholesterol
Commercial species
Hypersensitivity
Immunization
Immunology
Life Sciences
Medical Microbiology
Microbiology
Pharmacology
Polyunsaturated fatty acids
Ulva
Apply Biochemistry
Triterpene Glycoside
Triterpene
Saponin
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Summary:A novel antigen carrier has been formulated on the basis of a cucumarioside-A₂-2 triterpene glycoside (CD) complex with cholesterol and monogalactosyldiacylglycerol from Ahnfeltia tobuchiensis (MGDGAt) and Ulva fenestrate (MGDGUf). Morphological and immunostimulative characteristics of the carrier were studied. Electron microscopy experiments demonstrated the formation of homogeneous tubular structures in a mixture of CD, cholesterol, and MGDG in molar ratio of 1: 2: 3. In animals immunized by the carrier bearing pore forming protein monomer of pseudotuberculosis agent CD and MGDG synergically affected synthesis of specific antibodies, interleukin-2, and γ-interferon and delayed hypersensitivity reaction when compared to Freund's complete adjuvant or to immunostimulatory complexes between Quillaja saponaria saponins and phosphatidylcholine from egg yolk. The immunostimulatory effect depends upon the composition of polyunsaturated fatty acids of MGDG. The new tubular adjuvant carrier is a competitive adjuvant, as it includes CD obtained from far-eastern sea cucumber commercial species Cucumaria japonica, and MGDG from seaweed.
Bibliography:http://dx.doi.org/10.1134/S0003683808060124
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683808060124