Chronic consumption of imbalance diets high in sucrose or fat induces abdominal obesity with different pattern of metabolic disturbances and lost in Langerhans cells population

• Published in: Life sciences (1973) Vol. 336; p. 122305
• Main Authors: Márquez Álvarez, Corazón de María, Gómez-Crisóstomo, Nancy P, De la Cruz-Hernández, Erick N, El-Hafidi, Mohammed, Pedraza-Chaverri, José, Medina-Campos, Omar Noel, Martínez-Abundis, Eduardo
• Format: Journal Article
• Language: English
• Published: Netherlands 01.01.2024
• Subjects: Animals; Blood Glucose - metabolism; Diabetes Mellitus, Type 2 - etiology; Diet; Diet, High-Fat - adverse effects; Fatty Acids, Nonesterified; Hyperglycemia; Langerhans Cells - metabolism; Male; Obesity - metabolism; Obesity, Abdominal - etiology; Rats; Rats, Wistar; Sucrose; Triglycerides - metabolism; Obesity; Glucose regulation; Beta cells death; Glucotoxicity; Lipotoxicity; Type two diabetes models
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2023.122305

Obesity is a worldwide health issue, associated with development of type 2 Diabetes Mellitus. The aim of this study is to analyze the effect of consumption of two hypercaloric diets on metabolic disturbance and beta cells damage. Male Wistar rats were subjected to twelve months consumption of three diets: a Control balanced diet (CTD, carbohydrates 58 %, proteins 29 %, lipids 13 %) and two hypercaloric diets, high in sucrose (HSD, carbohydrates 68 %, proteins 22 %, lipids 10 %) or high in fat (HFD, carbohydrates 31 %, proteins 14 %, lipids 55 %). Serum levels of glucose, triglycerides and free fatty acids were measured after zoometric parameters determination. Antioxidant enzymes activity and oxidative stress-marker were measured in pancreas tissue among histological analysis of Langerhans islets. Although diets were hypercaloric, the amount of food consumed by rats decreased, resulting in an equal caloric consumption. The HSD induced hypertriglyceridemia and hyperglycemia with higher levels in free fatty acids (FFA, lipotoxicity); whereas HFD did not increased neither the triglycerides nor FFA, nevertheless the loss of islets' cell was larger. Both diets induced obesity with hyperglycemia and significant reduction in Langerhans islets size. Our results demonstrate that consumption of HSD induces more significant metabolic disturbances that HFD, although both generated pancreas damage; as well hypercaloric diet consumption is not indispensable to becoming obese; the chronic consumption of unbalanced diets (rich in carbohydrates or lipids) may lead to abdominal obesity with metabolic and functional disturbances, although the total amount of calories are similar.