Comparison of three methods for pharmacokinetic screening in rats using LC-MS-MS

The influence of sample preparation on ion suppression is studied using the model compounds ropivacaine and 3OH-ropivacaine. Three sample preparation techniques are assessed: Solid Phase Extraction (SPE), Direct Dilution and Protein Precipitation. Although requiring more method development and being...

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Bibliographic Details
Published inChromatographia Vol. 55; no. S1; pp. S35 - S39
Main Authors Briem, S, Eklund, E, Williams, G, Brunvoll, J
Format Journal Article
LanguageEnglish
Published Vieweg Verlag 2002
Subjects
liquid chromatography
pharmacokinetics
rats
screening
solid phase extraction
tandem mass spectrometry
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Summary:The influence of sample preparation on ion suppression is studied using the model compounds ropivacaine and 3OH-ropivacaine. Three sample preparation techniques are assessed: Solid Phase Extraction (SPE), Direct Dilution and Protein Precipitation. Although requiring more method development and being more time-consuming than the two alternative preparation techiques, SPE had a greater impact on reducing ion suppression.
Bibliography:http://dx.doi.org/10.1007/BF02493350
ISSN:0009-5893
1612-1112
DOI:10.1007/BF02493350