An efficient micropropagation protocol for Vernonia amygdalina Delile - An economically valuable shrub

A micropropagation protocol was standardized for the Vernonia amygdalina from shoot tip explants. Multiple shoots were induced using shoot tip explants inoculated on Murashige and Skoog (MS) medium fortified with cytokinins (BAP, TDZ, and Kinetin) and auxin (NAA). MS medium containing BAP (4.4 µM) a...

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Bibliographic Details
Published inJournal of herbs, spices & medicinal plants Vol. 26; no. 3; pp. 267 - 274
Main Authors Joshi, Bhavana, Bhandari, Aakriti, Panwar, G.S.
Format Journal Article
LanguageEnglish
Published Philadelphia Taylor & Francis 02.07.2020
Taylor & Francis Ltd
Subjects
Acclimatization
Auxins
Cytokinins
Explants
In vitro
Indoleacetic acid
Kinetin
medicinal plant
Micropropagation
Plantlets
Rooting
shoot tip
Shoots
Vernonia amygdalina
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Summary:A micropropagation protocol was standardized for the Vernonia amygdalina from shoot tip explants. Multiple shoots were induced using shoot tip explants inoculated on Murashige and Skoog (MS) medium fortified with cytokinins (BAP, TDZ, and Kinetin) and auxin (NAA). MS medium containing BAP (4.4 µM) and NAA (1.59 µM) was the most optimal medium with 98.89% of shoot formation. Well-developed in vitro regenerated shoots were transferred into rooting media containing different concentrations of auxins (IAA, IBA, and NAA). 100% rooting was achieved in half-strength MS medium fortified with IBA (4.9 µM), with an average of 20.2 roots per shoot. In vitro developed plantlets were moved to polybag and transferred to greenhouse for better acclimatization.
ISSN:1049-6475
1540-3580
DOI:10.1080/10496475.2020.1728601