In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype

Treatment differences were observed in the in vitro adventitious shoot regeneration response from internodal explants of three genotypes of Populus deltoides cultured on media supplemented with five concentrations each of the cytokinins 6-benzyladenine, 2-isopentyladenine, and zeatin. For each of th...

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Bibliographic Details
Published inPlant cell reports Vol. 8; no. 8; pp. 459 - 462
Main Authors Coleman, G.D, Ernst, S.G
Format Journal Article
LanguageEnglish
Published Germany 01.12.1989
Subjects
benzyladenine
culture media
explants
genetic factors
genotype
in vitro
isopentenyladenine
laboratory techniques
Populus deltoides
regeneration
shoots
zeatin
Nebraska
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Summary:Treatment differences were observed in the in vitro adventitious shoot regeneration response from internodal explants of three genotypes of Populus deltoides cultured on media supplemented with five concentrations each of the cytokinins 6-benzyladenine, 2-isopentyladenine, and zeatin. For each of the three genotypes, the greatest number of shoots were consistently regenerated on media containing the cytokinin zeatin. Tissue necrosis resulted when explants from any of the three genotypes were cultured on media supplemented with 6-benzyladenine. A zeatin concentration by genotype interaction was also observed. Genotypic differences in shoot regeneration were observed for 16 genotypes of Populus deltoides when cultured on medium supplemented with 0.5 mgL(-1) zeatin. Six genotypes were highly recalcitrant and failed to regenerate shoots. The percent of explants regenerating was greater than 50% for four genotypes.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00269048