Isolation and characterization of soil strains producing glutaryl-7-aminocephalosporanic acid acylase

A search was undertaken to screen microorganisms that produce an enzyme capable of deacylating glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid in soil samples. The screening was carried out by preparing enrichment cultures containing glutaryl-7ACA and cephalosporin C as selective...

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Published inBiotechnology and bioprocess engineering Vol. 2; no. 2; pp. 105 - 108
Main Authors Khang, Yong-Ho, Yoo, Byong-Hoon
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.12.1997
Subjects
Acidic soils
Amides
Aminocephalosporanic acid
Bacteria
Carbon sources
Cephalosporin C
Environmental conditions
Enzymes
Fermentation
Glycerol
Liquor
Microbiology
Microorganisms
pH effects
Screening
Sodium
Soil
Soils (acid)
Strains (organisms)
Substrates
Yeast
β-Lactam antibiotics
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Summary:A search was undertaken to screen microorganisms that produce an enzyme capable of deacylating glutaryl-7-aminocephalosporanic acid to 7-aminocephalosporanic acid in soil samples. The screening was carried out by preparing enrichment cultures containing glutaryl-7ACA and cephalosporin C as selective carbon sources. A non-β-lactam model compound, glutaryl-p-nitroanilide, was synthesized as a substrate suitable for the rapid screening of microorganisms isolated from the enrichment cultures. Two isolates exhibiting acylase activity, designated BY7.4 and BY8.1, were identified as strains ofPseudomonas species.Pseudomonas BY8.1 showed higher acylase activity toward Gl-7ACA thanPseudomonas BY7.4. Environmental conditions for the optimal acylase activity ofPseudomonas BY8.1 were shown to be pH 9 and 30°C.[PUBLICATION ABSTRACT]
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:1226-8372
1976-3816
DOI:10.1007/BF02932335