Evaluation of the histopathological effects of injectable hyaluronic acid as a filler material in rat’s lip

The present work aimed to examine the early and late histopathological changes occurring in the lower lips of rats, as a result of hyaluronic acid injection, and to determine whether it could really bring us closer to the ideal soft tissue filler material. This research highlights the importance of...

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Published inJournal of oral and maxillofacial surgery, medicine, and pathology Vol. 36; no. 6; pp. 885 - 894
Main Authors Al-aroomy, Leena, AbdelBary, Sawsan, El-Rouby, Dalia, Alwadeai, Mohamed, Ashoub, Madiha
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.11.2024
Subjects
Filler material
Histopathological effect
Hyaluronic acid
Immunohistochemical
Immunohistochemical
VEGF
Filler material
Histopathological effect
TGF- β
ECM
NASHA
HMW
T LR
GFs
LMW
HA
Hyaluronic acid
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Summary:The present work aimed to examine the early and late histopathological changes occurring in the lower lips of rats, as a result of hyaluronic acid injection, and to determine whether it could really bring us closer to the ideal soft tissue filler material. This research highlights the importance of experimental studies on this subject, since adverse reactions have been observed routinely in dental practice. The present study was carried out on 36 adult male albino rats and was divided into two equal groups, each consisting of 18 rats; group I (control group) and group II (experimental group). Rats in the experimental group were injected with hyaluronic acid (0.07 ml), whereas rats in the control group were injected with 0.9% sodium chloride. The results showed inflammation triggered by the injection of hyaluronic acid. On examining the specimens histologically using Hematoxylin and Eosin stains, Toluidine Blue stain and immunohistochemistry with anti-CD68 antibody, there was a significantly greater inflammatory response in the groups at early interval with hyaluronic acid compared with the control (P < 0.0001). Higher collagen formation was also observed with Masson’s Trichrome staining. The undesirable effects of hyaluronic acid filler injection can be detected at early intervals following its injection. Fortunately, all the undesirable effects are transient and decrease by time, reaching levels comparable to normal. Overall, HA was well-tolerated by tissues, reflecting how this filler material possesses a biocompatible property.
ISSN:2212-5558
DOI:10.1016/j.ajoms.2024.03.006