Polymorphism of the SLC25A30 gene in Indonesian sheep and its association with carcass characteristics

• Published in: BIO Web of Conferences Vol. 81; p. 29
• Main Authors: Makmur, Fajriani Mutmainnah, Listyarini, Kasita, Noor, Ronny Rachman, Damayanthi, Evy, Gunawan, Asep
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 01.01.2023
• Subjects: Carcasses; Gene polymorphism; Genetic improvement; Genotype & phenotype; Genotypes; Meat; Polymorphism; Restriction fragment length polymorphism; Sheep; Single-nucleotide polymorphism
• ISSN: 2117-4458; 2273-1709; 2117-4458
• DOI: 10.1051/bioconf/20238100029

Carcass characteristics are one of the factors that significantly impact the quality of meat. Genetic improvement in carcass characteristics is required to encourage interest among consumers. The objective of this study was to discover the presence of the SLC25A30 gene polymorphism at SNP g.15912281 A>T and its potential association with carcass characteristics in Indonesian sheep. The study used 153 rams, consisting of 87 Javanese thin-tail sheep, 20 Javanese fat-tail sheep, 10 Garut composite sheep, 10 Compass Agrinak sheep, 10 Barbadoss cross sheep and 16 jonggol sheep. The PCR-RFLP method was used to investigate the SLC25A30|SspI polymorphism. The association between the SLC25A30 gene and carcass characteristics was analyzed using the T-Test method. The results showed that the SLC25A30 (g.15912281 A>T) gene polymorphism was polymorphic in all breeds. The genotypes identified in the polymorphism were AA, AT and TT. The SLC25A30 gene was significantly associated (P<0.05) with carcass characteristics (slaughter weight, hot carcass, carcass percentage, carcass length and cold carcass). In conclusion, the genotype that can be recommended for selecting good meat quality is the TT genotype respectively than the AT and AA genotype The SLC25A30 (g.15912281 A>T) gene indicates as a prospective gene for the purpose of identifying carcass characteristics in lamb meat.