A Water Polysaccharide–Protein Complex from Grifola frondosa Inhibit the Growth of Subcutaneous but Not Peritoneal Colon Tumor under Fasting Condition

• Published in: Molecular nutrition & food research Vol. 68; no. 15; pp. e2400023 - n/a
• Main Authors: Xu, Yongzhao, Huang, Chujun, Xu, Tianxiong, Xiang, Xiong, Amakye, William Kwame, Zhao, Zikuan, Yao, Maojin, Zhu, Ying, Ren, Jiaoyan
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.08.2024
• Subjects: Apoptosis; Autophagy; Cell migration; Colon cancer; Dietary restrictions; Fasting; Fatty acid composition; Fatty acids; G. frondosa PPC; Gaylussacia frondosa; Grifola frondosa; Lipids; Molecular weight; nutrition restriction; polysaccharide; Polysaccharides; Proteins; Tumor microenvironment; Tumors; G. frondosa PPC; nutrition restriction; tumor microenvironment; fatty acids; polysaccharide; colon cancer
• ISSN: 1613-4125; 1613-4133; 1613-4133
• DOI: 10.1002/mnfr.202400023

Scope Grifola frondosa has been shown to induce immune modulatory, modulate autophagy, and apoptosis in cancer cells. However, little is known about its potential for managing tumor progression as an adjunct to nutrient restriction. Methods and results Water extract produces a G. frondosa polysaccharide–protein complex (G. frondosa PPC) of average molecular weight of 46.48 kDa, with glucose (54.8%) as the main constituent. Under serum‐restricted conditions, G. frondosa PPC can significantly inhibit MC38 colorectal tumor cell migration in vitro. Under alternate‐day fasting condition, G. frondosa PPC can only significantly inhibit the growth of subcutaneous (s.c.) tumor, but is feeble in halting its spread in the intraperitoneal (i.p.) cavity in tumor‐bearing mice. Histopathological examination and Raman imaging show a significant increase in lipid content in the tumor microenvironment (TME) tissue of the s.c. tumor‐bearing mice. G. frondosa PPC significantly increases C17:0 and C24:0 saturated fatty acids and significantly decreases C16:1 and C18:1 monounsaturated fatty acids in the TME of s.c. tumor‐bearing mice compared with the i.p. cavity model. Conclusion G. frondosa PPC significantly inhibits tumor growth in s.c. tumor‐bearing mice under intermittent fasting conditions by altering the fatty acid composition of the TME. Under alternate‐day fasting condition, G. frondosa PPC can only significantly inhibit the growth of (s.c.) tumor, but is feeble in halting its spread in the i.p. cavity in tumor‐bearing mice. G. frondosa PPC significantly inhibits tumor growth in s.c. tumor‐bearing mice under intermittent fasting conditions by altering the fatty acid composition of the tumor microenvironment.