Selective recovery of striatal 125I-α-conotoxinmii nicotinic receptors after nigrostriatal damage in monkeys

• Published in: Neuroscience Vol. 127; no. 2; pp. 399 - 408
• Main Authors: Lai, A, Sum, J, Fan, H, McIntosh, J.M, Quik, M
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2004; Elsevier
• Subjects: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; A85380; Animals; Azetidines - pharmacology; Biological and medical sciences; Bridged Bicyclo Compounds, Heterocyclic - metabolism; Bridged Bicyclo Compounds, Heterocyclic - pharmacology; Conotoxins - metabolism; Conotoxins - pharmacokinetics; Corpus Striatum - metabolism; Corpus Striatum - physiopathology; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Disease Models, Animal; Dopamine Plasma Membrane Transport Proteins; epibatidine; Female; Fundamental and applied biological sciences. Psychology; Iodine Radioisotopes; Male; Medical sciences; Membrane Glycoproteins - metabolism; Membrane Transport Proteins - metabolism; Mice; Mice, Inbred C57BL; MPTP; Nerve Tissue Proteins - metabolism; Neural Pathways - metabolism; Neural Pathways - physiopathology; Neurology; nigrostriatal; Parkinson; Parkinsonian Disorders - chemically induced; Parkinsonian Disorders - metabolism; Parkinsonian Disorders - physiopathology; Presynaptic Terminals - drug effects; Presynaptic Terminals - metabolism; Pyridines - metabolism; Pyridines - pharmacology; Receptors, Nicotinic - drug effects; Receptors, Nicotinic - metabolism; Recovery of Function - physiology; Saimiri; Substantia Nigra - metabolism; Substantia Nigra - physiopathology; Up-Regulation - drug effects; Up-Regulation - physiology; Vertebrates: nervous system and sense organs; α-conotoxinMII; BSA; A85380; α-conotoxinMII; epibatidine; nigrostriatal; MPTP; EDTA; Parkinson; EGTA; Nervous system diseases; Central nervous system; Monkey; Parkinson disease; Basal ganglion; Corpus striatum; Cerebral disorder; Toxin; Cholinergic receptor; Vertebrata; Mammalia; Central nervous system disease; Neurotoxin; Primates; Degenerative disease; Nicotinic receptor; Nigrostriatal pathway; Extrapyramidal syndrome
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2004.04.059

Evidence suggests that nicotinic receptors play a role in nigrostriatal function, a finding that may be relevant to Parkinson's disease. Knowledge of the conditions that regulate nicotinic receptor expression is therefore important. Previous studies showed that several different nicotinic receptors, including α-conotoxinMII (α-CtxMII)-sensitive receptors, are decreased after nigrostriatal damage. Nigrostriatal dopaminergic terminals also demonstrate a capacity for recovery after lesioning. The present experiments were therefore done to determine whether there were changes in striatal nicotinic receptors with recovery. To address this, we used two well-characterized animal models of nigrostriatal damage produced using the selective dopaminergic neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). Studies in mice showed that striatal 125I-α-CtxMII, as well as 125I-epibatidine and 125I-A85380 binding sites significantly recovered 1 month after lesioning, suggesting that α6* and most likely α4* receptors are increased. Experiments were next done in monkeys since striatal 125I-α-CtxMII receptors constitute a large percentage of nicotinic receptors and are more vulnerable to nigrostriatal damage in this model that closely mirrors Parkinson's disease. In monkeys allowed to recover from the toxic effects of MPTP for a 1–2 year period, there was a significant improvement in the Parkinson disability score. There was also a reversal in lesion-induced declines in striatal α-CtxMII-sensitive receptors, but no significant change in 125I-epibatidine and 125I-A85380 receptors. These findings suggest that α3*/α6* sites are selectively increased in monkey striatum with recovery. The present data show that recovery of 125I-α-CtxMII receptors occurs in parallel with the dopamine transporter, indicating that these nicotinic receptors sites are localized to presynaptic dopamine terminals in both species.