Induction of embryogenesis in cultured mature zygotic embryos of Abies fraseri (Pursh) Poir

• Published in: Plant cell, tissue and organ culture Vol. 49; no. 3; pp. 219 - 222
• Main Authors: Guevin, T.G, Kirby, E.G
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1997
• Subjects: 2,4-D; Abies fraseri; abscisic acid; benzyladenine; Biological and medical sciences; Biotechnology; Christmas trees; culture media; dose response; embryo culture; embryogenesis; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro propagation: entire plant regeneration from tissues and cell cultures; indole butyric acid; induction; isopentenyladenine; methodology; Methods. Procedures. Technologies; micropropagation; naphthaleneacetic acid; Plant cells and fungal cells; regenerative ability; thidiazuron; zygote; Culture medium; Embryonic development; Purine derivatives; Vegetals; Tissue culture; Cytokinin; Somatic embryo; Regeneration; Abies fraseri; Embryo culture; Gymnospermae; Softwood forest tree; Ureas; Plant growth substance; Coniferales; Spermatophyta
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1023/A:1005747026269

Embryo suspensor masses were induced by culture of isolated mature zygotic embryos of Fraser fir (Abies fraseri [Pursh] Poir.). Maximum induction frequencies were observed after 10 weeks culture on one-half strength Murashige and Skoog medium containing 10 micromolar thidiazuron and on one-half strength Verhagen and Wann medium containing 10 microgram cytokinin [6-(dimethylallylamino)purine, 6-benzyladenine, or thidiazuron). Proliferation of embryo suspensor masses occurred on one-half strength Verhagen and Wann medium supplemented with 10 micromolar cytokinin. When embryo suspensor masses were transferred to media containing 5-80 micromolar abscisic acid, cotyledonary-stage embryos were formed. Somatic embryos germinated on medium lacking plant growth regulators, but abnormal cotyledonary-stage somatic embryos with stunted cotyledons and reduced embryo axes were also observed.