Rapid Detection of Listeria in Ice Cream in 13 Hours Using the Roka Listeria Detection Assay
contamination is a major concern in the ice cream industry; therefore, early and accurate detection is vital. Current detection methods require about a 24 h enrichment period for detection. Enhance the early detection of in ice cream using the highly sensitive isothermal ribosomal RNA-based Roka/Atl...
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Published in | Journal of AOAC International Vol. 101; no. 6; pp. 1806 - 1812 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.11.2018
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Subjects | |
Online Access | Get full text |
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Summary: | contamination is a major concern in the ice cream industry; therefore, early and accurate detection is vital. Current detection methods require about a 24 h enrichment period for detection.
Enhance the early detection of
in ice cream using the highly sensitive isothermal ribosomal RNA-based Roka/Atlas
Detection Assay.
The R2 Medium was developed for
enrichment by Molecular Epidemiology, Inc. (Seattle, WA). Comparative growth curve studies were performed on the new R2 Medium for
and the currently validated media for the Roka
Detection Assay. Subsequently, a method comparison between the Roka
Detection Assay and the U.S. Food and Drug Administration's (FDA)
(BAM) Chapter 10 reference method on ice cream was carried out.
The R2 Medium supports the growth of
better than Buffered
Enrichment Broth, Demi-Fraser broth, and Modified University of Vermont Broth, as indicated by the faster growth rate of the organism. When used as an enrichment medium in a method comparison study of ice cream, the results showed that R2 Medium-enriched samples tested with the Roka
Detection Assay gave an equivalent performance compared with the 24 h FDA-BAM reference method at 10 and 18 h post-enrichment for
.
The results from this study indicate that the new R2 Medium and the highly sensitive Roka
Detection Assay allowed for the rapid detection of
species in ice cream in 13 h.
The Roka
Detection Assay, in conjunction with a new media formulation (R2 Medium), allowed for the early detection of
in ice cream and may be applied in other food matrixes and environmental samples. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 |
ISSN: | 1060-3271 1944-7922 |
DOI: | 10.5740/jaoacint.18-0108 |