LCHP Diet Enriched with Cholesterol Promotes Non-Alcoholic Fatty Liver Disease in Wistar Rats

The aim of this study was to analyze the effect of a low-carbohydrate–high-protein diet, enriched with cholesterol (LCHPch), on body weight, lipid metabolism, as well as kidney and liver function in rats. Wistar rats (N = 18, male) were randomly allocated into experimental groups and fed a modified...

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Published inApplied sciences Vol. 12; no. 16; p. 8266
Main Authors Kostogrys, Renata B., Franczyk-Żarów, Magdalena, Kus, Edyta, Topolska, Kinga
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.08.2022
Subjects
Abdomen
Adipose tissue
Animal tissues
Animals
Body fat
Body weight
Carbohydrates
Cardiovascular disease
Cholesterol
Diabetes
Diet
Experiments
Fatty liver
Fractions
High carbohydrate diet
High density lipoprotein
High protein diet
Kidneys
LCHP diet
Lipid metabolism
Lipids
Liver
Liver diseases
Low carbohydrate diet
Low protein diet
Metabolism
NAFLD
Nutrient deficiency
Obesity
Proteins
rat
Rodents
Poland
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Summary:The aim of this study was to analyze the effect of a low-carbohydrate–high-protein diet, enriched with cholesterol (LCHPch), on body weight, lipid metabolism, as well as kidney and liver function in rats. Wistar rats (N = 18, male) were randomly allocated into experimental groups and fed a modified AIN-93G diet with the addition of 1% cholesterol (AINch, WDch and LCHPch diets) for 8 weeks. Despite the lack of significant differences in the final body weight and liver weight of animals, the kidneys of rats in the LCHPch group were considerably heavier compared to the control group. Serum total cholesterol and the sum of low- and very-low-density fractions of cholesterol as well as ALT activity were significantly increased in the LCHPch rats in comparison to the AINch group. Simultaneously, the highest content of liver fat was obtained for animals from LCHPch group. It was also shown that both WDch and LCHPch diets significantly changed fatty acid profile in the adipose tissue of rats compared to control, with a significant increase in SFA and MUFA and a decrease in PUFA. Non-alcoholic fatty liver disease was observed in rats fed not only WDch but also LCHPch diets. The detailed mechanism still needs to be investigated to prevent the organism from the harmful effects of macronutrient dietary imbalance.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12168266