Spores germination and gametophytes of Alsophila odonelliana (Cyatheaceae) in different sterile media

• Published in: Phyton (Buenos Aires) Vol. 82; no. 1; pp. 119 - 126
• Main Authors: MC, Bonomo, Mart韓ez, OG, Tanco, ME, Cardozo, R, Avil閟, Z
• Format: Journal Article
• Language: English
• Published: Buenos Aires Tech Science Press 2013
• Subjects: 2,4-D; Alsophila; Culture media; Embryonic growth stage; Ferns; Gametangia; Gametophytes; Germination; Growth regulators; Somatic embryogenesis; Spore germination; Spores; Supplements
• ISSN: 1851-5657; 0031-9457; 1851-5657
• DOI: 10.32604/phyton.2013.82.119

The tree fern Alsophila odonelliana is found from southern Bolivia to northwestern Argentina, in the Tucuman-Bolivian forests. Spore germination was tested in three sterile nutritive media: Dyer (D), Murashige and Skoog (MS) and Knudson (K). We also evaluated the effect on gametophyte development of the addition of two growth regulators, BAP and 2,4-D to the culture medium Dyer; 2,4-D was evaluated using two concentrations: 0.1 and 2.0 mg/L, and BAP using three concentrations, 0.01; 0.1 and 1.0 mg/L. We found no significant differences in the germination of spores among all three media. We noted that supplementing media with BAP affected the longitudinal growth of the filamentous gametophytes, and the proliferation of ramifications from early stages. Supplementation with 2,4-D led to the development of short filamentous gametophytes and ameristic laminar forms. Both regulators affected the structure of male gametangia. There was not 2,4-D somatic embryogenesis as in other plants.