Improvement of rice callus regeneration in the presence of NaCl

• Published in: Plant cell, tissue and organ culture Vol. 57; no. 1; pp. 3 - 11
• Main Authors: Lutts, S, Kinet, J.M, Bouharmont, J
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1999
• Subjects: abscisic acid; application rate; Biological and medical sciences; Biotechnology; callus; cultivars; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; In vitro propagation: entire plant regeneration from tissues and cell cultures; indole acetic acid; interactions; Methods. Procedures. Technologies; mortality; Oryza sativa; Plant cells and fungal cells; polyethylene glycol; proline; regenerative ability; rooting; salt tolerance; sodium chloride; tissue culture; tryptophan; Monocotyledones; Vegetals; Salt resistance; Tissue culture; Environmental factor; Experimental protocol; Optimization; Cereal crop; Oryza sativa; Regeneration; Gramineae; Angiospermae; Spermatophyta; Sodium Chlorides
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1023/A:1006284310077

The effects of abscisic acid (37.8 micromolar), polyethylene glycol (5%), proline (10 mM), tryptophan (490 micromolar) and indoleacetic acid (5.7 micromolar) on rice callus regeneration were studied at various doses of NaCl (0, 50 and 100 mM) on three month-old mature embryo-derived callus of two japonica (I Kong Pao and Aiwu) and two indica (IR 2153 and Nona Bokra) rice cultivars differing in salinity tolerance. NaCl strongly decreased the regeneration frequency of all cultivars but slightly increased the survival of regenerated plantlets. Tryptophan stimulated regeneration and increased subsequent survival rates of regenerated plantlets in all cultivars at all NaCl doses. Abscisic acid and polyethylene glycol, though not affecting the final regeneration percentages, delayed regeneration and reduced the mean number of plantlets produced per regenerating callus in all cultivars, as well as rooting ability and survival of regenerated plantlets in indica genotypes. Proline had no marked effect on regeneration, whatever the NaCl dose or cultivar, while indoleacetic acid reduced shoot regeneration and increased root regeneration.