Identification of DR75-RS11090 Gene Encoding GGDEF Domains, and its Role in Enterococcus faecalis
Background: Enterococcus faecalis is considered the primary pathogenic bacteria in failed root canal therapy due to its excellent biofilm formation ability. The second messenger cyclic dimeric (3’→5’) GMP (c-di-GMP) is involved in bacterial physiological processes, including biofilm formation. C-di-...
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Published in | Jundishapur journal of microbiology Vol. In Press; no. In Press; pp. 1 - 7 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Ahvaz
Ahvaz Jundishapur University of Medical Sciences
01.01.2019
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Subjects | |
Online Access | Get full text |
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Summary: | Background: Enterococcus faecalis is considered the primary pathogenic bacteria in failed root canal therapy due to its excellent biofilm formation ability. The second messenger cyclic dimeric (3’→5’) GMP (c-di-GMP) is involved in bacterial physiological processes, including biofilm formation. C-di-GMP is formed by diguanylate cyclases with the GGDEF domain.Objectives: The current study aimed at investigating the function of DR75-RS11090 in E. faecalis.Methods: In the current experimental study, gene encoding GGDEF domains in E. faecalis were analyzed through National Center for Biotechnology Information (NCBI) database. Enterococcus faecalis strains were cultured in brain-heart-infusion (BHI) broth at 37°C. Growth curves of E. faecalis were measured in an automatic microplate reader. Biofilms were fixed with 2% glutaraldehyde and scanned under the transmission electron microscopy. Acid and water-insoluble polysaccharide productions were also detected by the precise digital acidometer and anthrone method.Results: It was found that DR75-RS11090 gene was predicted to encode GGDEF domain in E. faecalis. The current study observed promoting effects of DR75-RS11090 on E. faecalis biofilm formation, growth, acid production, and water insoluble polysaccharide production.Conclusions: The current study findings improved the understanding of c-di-GMP in E. faecalis and presented a primary study on DR75-RS11090 promoting mechanism. |
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ISSN: | 2008-3645 2008-4161 |
DOI: | 10.5812/jjm.83394 |