Isolation of pluripotent stem cells from human third molar dental pulp

• Published in: Histology and histopathology Vol. 26; no. 8; p. 1057
• Main Authors: Atari, M, Barajas, M, Hernández-Alfaro, F, Gil, C, Fabregat, M, Ferrés Padró, E, Giner, L, Casals, N
• Format: Journal Article
• Language: English
• Published: Spain 01.08.2011
• Subjects: Adolescent; Adult; Biomarkers - metabolism; Cell Differentiation; Cells, Cultured; Dental Pulp - cytology; Dental Pulp - metabolism; Female; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Induced Pluripotent Stem Cells - ultrastructure; Male; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells - ultrastructure; Microscopy, Electron, Scanning; Middle Aged; Molar, Third; Tissue Scaffolds; Young Adult
• ISSN: 1699-5848
• DOI: 10.14670/HH-26.1057

Potent stem/progenitor cells have been isolated from normal human dental pulps, termed dental pulp stem cells (DPSCs). However, no study has described the presence of stem cell populations in human dental pulp from the third molar with embryonic phenotypes. The dental pulp tissue was cultured in media with the presence of LIF, EGF, and PDGF. In the present study, we describe a new population of pluripotent stem cells that were isolated from dental pulp (DPPSC). These cells are SSEA-4(+), Oct4(+), Nanog(+), FLK-1(+), HNF3beta(+), Nestin(+), Sox2(+), Lin28(+), c-Myc(+), CD13(+), CD105(+), CD3(-), CD45(-), CD90(low), CD29(+), CD73(low), STRO-1(low) and CD146(-). We have investigated by SEM analysis and q-RT-PCR the capacity of DPPSCs to 3D differentiate in vitro using the Cell Carrier 3D glass scaffold into tissues that have similar characteristics to embryonic mesoderm and endoderm layers. These data would support the use of these cells, which are derived from an easily accessible source and can be used in future regeneration protocols for many tissue types that differentiate from the three embryonic layers.