Single and combined application of bacteriophage and cinnamon oils against pathogenic Listeria monocytogenes in milk and smoked salmon

• Published in: International journal of food microbiology Vol. 421; p. 110797
• Main Authors: Maung, Aye Thida, Abdelaziz, Marwa Nabil Sayed, Noor Mohammadi, Tahir, Lwin, Su Zar Chi, El-Telbany, Mohamed, Zhao, Junxin, Wang, Chen, Lin, Yunzhi, Shen, Cunkuan, Zayda, Mahmoud, Masuda, Yoshimitsu, Honjoh, Ken-ichi, Miyamoto, Takahisa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 16.08.2024
• Subjects: Cinnamon oil; Listeria monocytogenes; Milk; Phage; Smoked salmon; Cinnamon oil; BHI; TSA; Smoked salmon; MIC; Listeria monocytogenes; SM buffer; MBC; L. monocytogenes; C.b; LB; C.c; MH; Phage; Milk
• ISSN: 0168-1605; 1879-3460; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2024.110797

Nowadays, the discovery of alternative natural antimicrobial substances such as bacteriophages, essential oils, and other physical and chemical agents is developing in the food industry. In this study, nine bacteriophages were isolated from various parts of raw chickens and exhibited lytic activities against L. monocytogenes and various Listeria spp. The characterization of phage vB_LmoS-PLM9 was stable at 4 to 50 °C and pH range from 4 to 10. Phage vB_LmoS-PLM9 had a circular, double-stranded genomic DNA with 38,345 bp having endolysin but no antibiotic resistance or virulence genes. Among the eight essential oils tested at 10 %, cinnamon bark, and cassia oils showed the strongest antilisterial activities. The combined use of phage vB_LmoS-PLM9 and cinnamon oils indicated higher efficiency than single treatments. The combination of phage (MOI of 10) and both cinnamon oils (0.03 %) reduced the viable counts of L. monocytogenes and inhibited the regrowth of resistant cell populations in broth at 30 °C. Furthermore, treatment with the combination of phage (MOI of 100) and cinnamon oil (0.125 %) was effective in milk, especially at 4 °C by reducing the viable count to less than lower limit of detection. These results suggest combining phage and cinnamon oil is a potential approach for controlling L. monocytogenes in milk. •Phage vB_LmoS-PLM9 showed the strongest lytic activity against L. monocytogenes.•Cinnamon bark and cassia oils exhibited strong antilisterial activities.•Phage plus cinnamon oil controlled L. monocytogenes, and prevented regrowth in milk but not smoked salmon.