An investigation on skin wound healing in mice with a taurine-chitosan gel formulation

• Published in: Amino acids Vol. 22; no. 2; pp. 187 - 198
• Main Authors: Değim, Z, Celebi, N, Sayan, H, Babül, A, Erdoğan, D, Take, G
• Format: Journal Article
• Language: English
• Published: Austria 2002
• Subjects: Animals; Antioxidants - administration & dosage; Biocompatible Materials; Chemistry, Pharmaceutical; Chitin - administration & dosage; Chitin - analogs & derivatives; Chitosan; Collagen - analysis; Disease Models, Animal; Drug Delivery Systems; Hydroxyproline - metabolism; Materials Testing; Mice; Microscopy, Electron; Skin Diseases - drug therapy; Skin Diseases - pathology; Taurine - administration & dosage; Tensile Strength - drug effects; Wound Healing - drug effects
• ISSN: 0939-4451
• DOI: 10.1007/s007260200007

The process of wound healing begins immediately following surface lesions or just after exposure to radiation, chemical agents or extreme temperatures. Taurine (2-aminoethane sulfonic acid), an amino acid containing sulfur, is found in almost all tissues in mammals, playing various important physio-logical roles in each organ. Taurine exhibits an antioxidant effect and is also known to have effects on cell proliferation, inflammation and collagenogenesis. Many antioxidants have been used to eliminate the negative effects of oxygen free radicals on wound healing. The objective of the present study was to examine the wound healing effect in mice of taurine-chitosan gel, which releases taurine slowly over a long time period. Fifty mM of taurine in 1.5% chitosan polymer (TAU-GEL) and 1.5% chitosan polymer (CHI-GEL) were applied to full thickness skin wounds of mice once a day for seven days. After seven days of treatment, lipid peroxide formation-malondialdehyde (MDA) and hydroxyproline (HPX) levels and the tensile strength of wound tissues were measured. All results were compared with those of the untreated control group (CONT). The structural alterations in the skin layers were also histologically investigated. It was found that locally administered TAU-GEL form significantly increased wound tensile strength by decreasing the MDA and increasing HPX levels. These results were supported by histological findings. All observations suggest that taurine gel may be effective in wound healing.