Comparative metabolomic analysis and antigenicity comparison of cow milk and enzymatically treated cow milk

• Published in: Journal of the science of food and agriculture Vol. 104; no. 1; pp. 536 - 545
• Main Authors: Liang, Xiaona, Diao, Enjie, Qian, Shiquan, Song, Huwei, Xiang, Xinran, Gou, Xiurong, Hu, Xiumin
• Format: Journal Article
• Language: English
• Published: London John Wiley and Sons, Limited 15.01.2024
• Subjects: Amino acids; Antigenicity; Cattle; Cow's milk; Dairy products; Immune system; Metabolic pathways; Metabolomics; Milk
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.12927

Abstract BACKGROUND Amino acids (AAs) are important protein building blocks that play a critical role in the function of the immune system. However, comprehensive comparative metabolomics and antigenicity analyses of cow milk (CM) and enzymatically treated CM are relatively scarce. This study analyzed the AAs in the CM and Flavourzyme‐treated milk groups (FT), and their antigenicity was also explored. RESULTS Overall, 50 AAs were detected in the CM and FT groups, with 23 significantly different AAs. The interaction network of these significantly different AAs was analyzed, and 34 significantly different metabolic pathways were found to be involved. It was also found that the antigenicity of the FT group was significantly reduced in comparison with that of the CM group. CONCLUSION These results enhance our understanding of AAs and antigenicity regarding CM and FT, and provide new ideas and directions for the development of high‐quality hypoallergenic dairy products. © 2023 Society of Chemical Industry.