Comparative evaluation of the essential oil terpenoids in the stem and leaf of Ziziphora clinopodioides in the regions of Almeh and Sojough of Golestan province, Iran

• Published in: Acta Periodica Technologica Vol. 2012; no. 43; pp. 283 - 291
• Main Authors: Ebrahimi, Pouneh, Mirarab-Razi, Akram, Biabani, Abbas
• Format: Journal Article
• Language: English
• Published: Faculty of Technology, Novi Sad 2012
• Subjects: Golestan Province; microwave-assisted hydrodistillation; terpenoid compounds; Ziziphora clinopodioides
• ISSN: 1450-7188; 2406-095X
• DOI: 10.2298/APT1243283E

Microwave-assisted hydrodistillation was used to isolate essential oil from the leaf and stem of Ziziphora clinopodioides collected in the flowering stage on two locations, Almeh and Sojogh, of the Golestan Province (Iran), in June of 2009. The total contents of monoterpene and sesquiterpene fractions (52.45% and 1.08%, respectively) in the leaf oil of Almeh plants were higher than those of Sojogh (46.64% and 0.12%, respectively). The essential oil of the stem of the plants from Sojogh was characterized by the presence of eight oxygenated monoterpenes (22.17%), while four oxygenated monoterpenes (11.15%), one monterpene hydrocarbon (2.71%), and one oxygenated sesquiterpene (0.21%) were found in the plants from the region of Almeh. The analysis of the essential oil of dried aerial parts showed the presence of oxygenated monoterpenes pulegone and menthol (the region of Sojogh) and pulegone, 1,8-cineol, D-neoisomenthol and chrysanthenone (the region of Almeh), as the main constituents. Also, chrysanthenone (9.75%), found as the second major component of the leaf of Z. clinopodioides of Almeh, was not identified as the oil component of the other region. The results obtained on the chemical composition of Z. clinopodioides oil of two regions from the Golestan Province revealed that in general, that there are some differences in the major components and their relative concentrations. This may be probably due to the different environmental and genetic factors, different chemotypes and the nutritional status of the plants, as well as other factors that can influence the oil composition. Mikrotalasno-potpomognuta hidrodestilacija je primenjena da bi se izolovala etericna ulja iz uzoraka lista i stabla biljke Ziziphora clinopodioides prikupljenih u fazi cvetanja na dve lokacije-Almeh i Sodzog u provinciji Golestan (Iran), u junu 2009. Ukupni sadrzaji monoterpenskih i seskviterpenskih frakcija u ulju lista biljaka iz Almeha (52,45% i 1,08%, respektivno) su visi nego kod biljaka iz Sodzoga (46,64% i 0,12%, respektivno). Etericno ulje iz stabla biljaka iz So dzoga sadrzi osam oksidovanih monoterpena (22,17%), dok su cetiri oksidovana monoterpena (11,15%), jedan monoterpenski ugljovodonik (2,71%), i jedan oksidovani seskviterpen (0,21%) nadjeni u biljkama iz regiona Almeh. Analiza etericnih ulja osusenih nadzemnih delova biljaka pokazala je prisustvo oksidovanih monoterpena, pulegona i mentola (Sodzog region) i pulegona, 1,8-cineola, D-neoizomentola i hrizantenona (Almeh region), kao glavnih sastojaka. Takodje, hrizantenon (9,75%), nadjen kao druga glavna komponenta ulja iz lista biljke Z. Clinopodioides iz Almeha, nije bio identifikovan u drugom regionu ili biljnoj vrsti. Rezultati dobijeni odredjivanjem sastava ulja biljke Z. clinopodioides iz dva regiona provincije Golestan pokazuju generalno da postoje izvesne razlike u glavnim komponentama i njihovim relativnim koncentracijama. Ove razlike verovatno poticu od razlicitih ekoloskih uslova i genetskih faktora, razlicitih hemotipova i nutricionog statusa biljaka, kao i drugih faktora koji mogu uticati na sastav ulja.