Identification and Pathogenicity of Rhizoctonia solani Isolates Causing Leaf and Stem Rot in Three-Leaf Ladybell

In 2020 and 2021, we surveyed diseases of three-leaf ladybell (Adenophora triphylla) plants grown in fields at two locations in Korea. During the disease surveys, severe leaf rot symptoms were observed on the young plants in Hongseong, and stem rot symptoms on the adult plants in Cheolwon. The incid...

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Published inSigmulbyeong yeon'gu Vol. 29; no. 4; pp. 377 - 383
Main Authors Kim, Wan-Gyu, Choi, Hyo-Won, Lee, Gyo-Bin, Cho, Weon-Dae
Format Journal Article
LanguageEnglish
Published 한국식물병리학회 01.12.2023
Hanrimwon Publishing Company
Subjects
adenophora triphylla
anastomosis group
leaf and stem rot
rhizoctonia solani
three-leaf ladybell
Leaf and stem rot
Rhizoctonia solani
Anastomosis group
Three-leaf ladybell
Adenophora triphylla
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Summary:In 2020 and 2021, we surveyed diseases of three-leaf ladybell (Adenophora triphylla) plants grown in fields at two locations in Korea. During the disease surveys, severe leaf rot symptoms were observed on the young plants in Hongseong, and stem rot symptoms on the adult plants in Cheolwon. The incidence of leaf rot was 5‒60%, and that of stem rot 1‒10%. We obtained 6 fungal isolates each from the leaf rot lesions and the stem rot lesions. All the isolates were morphologically identified as Rhizoctonia solani. Anastomosis test and investigation of cultural features of the fungal isolates revealed that the isolates from the leaf rot lesions corresponded to R. solani AG-1(IB), and those from the stem rot lesions to R. solani AG-2-2(IIIB). Two isolates each of R. solani AG-1(IB) and AG-2-2(IIIB) were used for DNA sequence analysis and pathogenicity test to three-leaf ladybell plants through artificial inoculation. The anastomosis groups and cultural types of the R. solani isolates were confirmed by the sequence analysis. The pathogenicity tests revealed that the isolates of R. solani AG- 1(IB) caused only leaf rot symptoms on the inoculated plants, and those of R. solani AG-2-2(IIIB) leaf rot and stem rot symptoms. The induced symptoms were similar to those observed in the fields investigated. Leaf and stem rot of three-leaf ladybell caused by the two anastomosis groups and cultural types of R. solani is first reported in this study.
ISSN:1598-2262
2233-9191
DOI:10.5423/RPD.2023.29.4.377