Lentilactobacillus hilgardii H-50 strongly inhibits lipopolysaccharide-induced inflammatory responses in mouse splenocytes via its specific surface layer proteins

• Published in: Journal of applied microbiology Vol. 134; no. 3
• Main Authors: Yamaguchi, Tomofumi, Yasui, Kenta, Fujii, Sotaro, Ichikawa, Kennosuke, Suzuki, Takuya, Sambongi, Yoshihiro, Yamamoto, Yoshinari
• Format: Journal Article
• Language: English
• Published: England 01.03.2023
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Lipopolysaccharides - pharmacology; Mice; Spleen - metabolism; Tumor Necrosis Factor-alpha - metabolism; Lipopolysaccharide; surface layer protein; Lactic acid bacteria; anti-inflammatory properties; Lentilactobacillus hilgardii
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxad021

Certain lactic acid bacteria (LAB) are known to have anti-inflammatory effects; however, hiochi bacteria, which are taxonomically classified as LAB and known to spoil a traditional Japanese alcoholic beverage, have not been studied in the same context. The aim of this study is to investigate the anti-inflammatory effects of hiochi bacteria strains and the underlying mechanisms. We screened 45 strains of hiochi bacteria for anti-inflammatory effects and found that Lentilactobacillus hilgardii H-50 strongly inhibits lipopolysaccharide (LPS)-induced secretion of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in mouse splenocytes. This inhibition is attributed to its specific surface layer proteins (SLPs), which directly bind to LPS. The L. hilgardii H-50 strain exerts anti-inflammatory effects through its SLPs.