Bacterial Translocation Ratchets: Shared Physical Principles with Different Molecular Implementations: How bacterial secretion systems bias Brownian motion for efficient translocation of macromolecules

• Published in: BioEssays Vol. 39; no. 10
• Main Authors: Hepp, Christof, Maier, Berenike
• Format: Journal Article
• Language: English
• Published: United States 01.10.2017
• Subjects: Bacterial Secretion Systems - metabolism; Bacterial Translocation - physiology; Biological Transport - physiology; Macromolecular Substances - metabolism; translocation ratchet; chemical potential; secretion system; transformation; Brownian ratchet; molecular motor; protein secretion
• ISSN: 0265-9247; 1521-1878
• DOI: 10.1002/bies.201700099

Secretion systems enable bacteria to import and secrete large macromolecules including DNA and proteins. While most components of these systems have been identified, the molecular mechanisms of macromolecular transport remain poorly understood. Recent findings suggest that various bacterial secretion systems make use of the translocation ratchet mechanism for transporting polymers across the cell envelope. Translocation ratchets are powered by chemical potential differences generated by concentration gradients of ions or molecules that are specific to the respective secretion systems. Bacteria employ these potential differences for biasing Brownian motion of the macromolecules within the conduits of the secretion systems. Candidates for this mechanism include DNA import by the type II secretion/type IV pilus system, DNA export by the type IV secretion system, and protein export by the type I secretion system. Here, we propose that these three secretion systems employ different molecular implementations of the translocation ratchet mechanism.