Influence of microbial fermentation on the antioxidant activity of phenolic substances in Saccharomyces cerevisiae

• Published in: Journal of applied microbiology Vol. 134; no. 8
• Main Authors: de Souza, Edlene Ribeiro Prudêncio, da Conceição Braz, Marcos Vinicius, Castro, Rosane Nora, Pereira, Marcos Dias, Riger, Cristiano Jorge
• Format: Journal Article
• Language: English
• Published: England 01.08.2023
• Subjects: CAPE; mangiferin; Saccharomyces cerevisiae; antioxidant activity; microbial fermentation
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxad148

Using in vitro assay and eukaryotic cell model of Saccharomyces cerevisiae, we investigated the impact of microbial fermentation on antioxidant activity of phenolic substances. Caffeic acid phenethyl ester (CAPE) and mangiferin were fermented by lactic acid bacteria (LAB), and the antioxidant activity of the fermented products was compared to that of the pure substances. This was assessed using HPLC and in vitro by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), and in vivo in yeast cells. The wild-type strain (BY4741) and its isogenic mutants in glutathione (Δgsh1), catalase (Δctt1), and superoxide dismutase (Δsod1) were treated with CAPE and mangiferin, fermented or not, and exposed to hydrogen peroxide (H2O2)-induced stress. The antioxidant activity was evaluated by cellular viability, intracellular oxidation and lipid peroxidation. We expected that fermentation would change the antioxidant activity of phenolic substances. While HPLC analysis revealed changes in the composition of fermented products, significant alterations in antioxidant activity were only observed when using mutant strains. The fermentation of mangiferin increased dependency on GSH compared to the respective pure phenolic substance to resolve H2O2-induced stress. Additionally, CAPE appeared to act as a preconditioning agent, enhancing antioxidant responses and promoting increased tolerance to H2O2 stress, and this mechanism was maintained after fermentation. This study highlights that fermentation impacts the enzymatic mechanism of oxidative stress resolution, even though differences could not be observed in in vitro assays or wild-type strain.