Aliidiomarina quisquiliarum sp. nov., isolated from landfill leachate

• Published in: International journal of systematic and evolutionary microbiology Vol. 73; no. 2
• Main Authors: Zhang, Xuan-qi, Xamxidin, Maripat, Li, Jing-hang, Zhang, Xin-yin, Wang, Tao, Chen, Can, Wu, Min, Ke, Han
• Format: Journal Article
• Language: English
• Published: England 01.02.2023
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Water Pollutants, Chemical; China; phylogenetic analysis; leachate; draft genome; Aliidiomarina; landfill
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijsem.0.005685

Bacterial strain Y-6 T , isolated from a landfill site in Yiwu, PR China, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed pale beige colonies. Strain Y-6 T grew at 4–40 °C (optimal at 30–37 °C), pH 6.5–9.5 (optimal at pH 7.2–8.5) and in the presence of 0.5–10.0 % (w/v) NaCl (optimal at 1.0–3.0 %). Phylogenetic analysis revealed that strain Y-6 T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis MCCC 1A06493 T with a 16S rRNA sequence similarity of 98.2 %. The major cellular fatty acids of the isolate were iso-C 15 : 0 , C 16 : 0 , iso-C 17 : 0 and summed feature 9 (iso-C 17 : 1  ω 9 c and/or 10-methyl-C 16 : 0 ). Q-8 was the predominant ubiquinone. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycophospholipid, aminophospholipid, phospholipid, three glycolipids and two unknown lipids. The genomic DNA G+C content was 46.6 mol%. The digital DNA–DNA hybridization value between Y-6 T and A. taiwanensis MCCC 1A06493 T was 18.3 %. Strain Y-6 T had an average nucleotide identity value of 74.09 % with A. taiwanensis MCCC 1A06493 T . Results from the polyphasic taxonomy study support the conclusion that strain Y-6 T represents a novel Aliidiomarina species, for which the name Aliidiomarina quisquiliarum sp.nov. is proposed. The type strain is Y-6 T (=MCCC 1K06228 T =KCTC 82676 T ).