Mumia quercus sp. nov., isolated from the root of Quercus variabilis

A novel endophytic actinomycete, designated strain NEAU-365 , was isolated from the root of collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365 belonged to the genus but clearly differs from the currently recognized species Z350 (99.31 %), NEAU...

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Published inInternational journal of systematic and evolutionary microbiology Vol. 73; no. 4
Main Authors Chen, Hongmin, Cao, Tingting, Wang, Teng, Gao, Congting, Yang, Jingquan, Dong, Qingyan, Shen, Yue, Liu, Chongxi
Format Journal Article
LanguageEnglish
Published England 01.04.2023
Subjects
Actinomycetales
Bacterial Typing Techniques
Base Composition
DNA, Bacterial - genetics
Fatty Acids - chemistry
Phospholipids
Phylogeny
Quercus
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Vitamin K 2
Quercus variabilis
Mumia quercus sp. nov
polyphasic taxonomy
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Summary:A novel endophytic actinomycete, designated strain NEAU-365 , was isolated from the root of collected from Nanjing, Jiangsu, PR China. Comparative 16S rRNA gene sequencing showed that strain NEAU-365 belonged to the genus but clearly differs from the currently recognized species Z350 (99.31 %), NEAU-KD1 (98.82 %) and MUSC 201 (97.78 %). Phylogenetic tree analysis revealed that strain NEAU-365 clustered with the type strains of the genus . The genome size was 4.1 Mbp with a DNA G+C content of 71.2 mol%. Digital DNA-DNA hybridization and average nucleotide identity values between the genome sequence of strain NEAU-365 and those of Z350 (27.5 and 84.0 %), NEAU-KD1 (23.4 and 80.9 %) and MUSC 201 (20.9 and 77.7 %) were below the recommended thresholds for species delineation. Cells were observed to be irregular cocci shaped. The cell wall contained ll-diaminopimelic acid and the whole-cell sugars were galactose and rhamnose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid and three unidentified phospholipids. The predominant menaquinone was detected as MK-9(H ). The major fatty acids were C , C , C  ω9 and 10-methyl C . On the basis of genotypic and phenotypic differences from members of the genus , a novel species, sp. nov. is proposed. The type strain is NEAU-365 (=CCTCC AA 2021033 =JCM 35005 ).
ISSN:1466-5034
DOI:10.1099/ijsem.0.005776