Histidyl-tRNA synthetase of Chinese hamster ovary cells contains phosphoserine

The Chinese hamster ovary cell line 2H2–5, which expresses elevated levels of histidyl-tRNA synthetase, was used to demonstrate that histidyl-tRNA synthetase contains phosphoserine. After growth of the cells in medium containing [ 32P]orthophosphate, histidyl-tRNA synthetase was isolated by partial...

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Published inBiochimica et biophysica acta, Protein structure and molecular enzymology Vol. 869; no. 2; pp. 215 - 217
Main Authors Gerken, Steven C., Andrulis, Irene L., Arfin, Stuart M.
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.01.1986
Subjects
aminoacyl-tRNA synthetase
CHO cells
hamster ovary
Histidyl-tRNA synthetase
Phosphorylation
Phosphoserine
Phosphorylation
Histidyl-tRNA synthetase
hamster ovary
aminoacyl-tRNA synthetase
Phosphoserine
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Summary:The Chinese hamster ovary cell line 2H2–5, which expresses elevated levels of histidyl-tRNA synthetase, was used to demonstrate that histidyl-tRNA synthetase contains phosphoserine. After growth of the cells in medium containing [ 32P]orthophosphate, histidyl-tRNA synthetase was isolated by partial purification and immunoprecipitation and shown to be a phosphoprotein. Phosphoamino acid analysis showed that histidyl-tRNA synthetase is phosphorylated on serine, as has previously been shown for threonyl-tRNA synthetase of CHO cells.
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ISSN:0167-4838
1879-2588
DOI:10.1016/0167-4838(86)90296-7