Winogradskyella bathintestinalis sp. nov., isolated from the intestine of the deep-sea loosejaw dragonfish, Malacosteus niger

• Published in: International journal of systematic and evolutionary microbiology Vol. 73; no. 10
• Main Authors: Uniacke-Lowe, Shona, Johnson, Crystal N, Stanton, Catherine, Hill, Colin, Ross, Paul
• Format: Journal Article
• Language: English
• Published: England 01.10.2023
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Fishes; Intestines; Niger; Perciformes; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Niger; Winogradskyella; fish isolate; gut microbiome; deep sea; sp. nov
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.006135

A novel bacterial strain, APC 3343 , was isolated from the intestine of a deep-sea loosejaw dragon fish, , caught at a depth of 1000 m in the Northwest Atlantic Ocean. Cells were aerobic, rod-shaped, yellow/orange-pigmented, non-motile and Gram-negative. Growth of strain APC 3343 was observed at 4-30 °C (optimum, 21-25 °C), pH 5.5-10 (optimum, pH 7-8) and 0.5-8 % (w/v) NaCl (optimum, 2-4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain APC 3343 was most closely related to members of the genus , with the most closely related type strains being Kr9-9 (98.46 % identity), F081-2 (98.07 %), CECT 7946 (97.93 %), KMM 6491 (97.79 %) and HL2-2 (97.79 %). Major fatty acids (>10 % of total) were iso-C 3-OH, iso-C , anteiso-C and iso-C 3-OH. The predominant respiratory quinone was menaquinone-6 (MK-6). Polar lipids were phosphatidylethanolamine, three unknown aminolipids and eight unknown lipids. The draft genome sequence was 3.8 Mb in length with a G+C content of 33.43 mol%. Based on the phenotypic characteristics and phylogenetic analysis, strain APC 3343 is deemed to be a novel species of the genus , and for which the name sp. nov. is proposed. The type strain of this species is APC 3343 (=DSM 115832 =NCIMB 15464 ).