Luteimonas galliterrae sp. nov., isolated from poultry farm soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 73; no. 2
• Main Authors: Shen, Lei, An, Miao-Miao, He, Xiang-Wei, Zhao, Guo-Zhu
• Format: Journal Article
• Language: English
• Published: England 01.02.2023
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Farms; Fatty Acids - chemistry; Phospholipids - chemistry; Phylogeny; Poultry; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil; Luteimonas galliterrae; genome analysis; 16S rRNA gene; polyphasic taxonomy
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.005717

Strain SX5 was isolated from the soil of a poultry farm in Shanxi Province, PR China. The isolate was a Gram-stain-negative, rod-shaped, non-flagellated, and yellow bacterium. Growth occurred at 20-37 °C (optimum, 28 °C), pH 6.0-10.0 (optimum, pH 8.0) and 0-1 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SX5 was related to members of the genus , and close to H23 (97.9 %), Y4 (97.9 %), RIB1-20 (96.8 %), SYP-B804 (96.4 %) and Gsoil 068 (96.1 %). The major cellular fatty acids of strain SX5 were iso-C , iso-C 9 , iso-C and iso-C 3OH. The sole isoprenoid quinone was ubiquinone Q-8, and the major polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Genome analyses revealed that strain SX5 had a genome size of 3.6 Mbp with a G+C content of 65.7 mol% and contained abundant carbohydrate-active enzyme genes and three putative distinct biosynthetic gene clusters, suggesting that it may have great potential to degrade and utilize complex biological organic matter and produce special secondary metabolites. Comparative genomic analyses clearly separated strain SX5 from the known species of the genus based on average nucleotide identity and digital DNA-DNA hybridization values below the thresholds for species delineation. Based on its phenotypic, genotypic properties and phylogenetic inference, strain SX5 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is SX5 (=GDMCC 1.2162 =KCTC 82443 =JCM 34401 ).