Terrirubrum flagellatum gen. nov., sp. nov. of Terrirubraceae fam. nov. and Lichenibacterium dinghuense sp. nov. from forest soil and proposal of Rhodoblastaceae fam. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 74; no. 4
• Main Authors: Guo, Xiu-Yin, Zhang, Qiu-Mei, Fu, Jia-Cheng, Qiu, Li-Hong
• Format: Journal Article
• Language: English
• Published: England 01.04.2024
• Subjects: Bacterial Typing Techniques; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids; Forests; Nucleic Acid Hybridization; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Ubiquinone; China; phylogeny; novel species; taxonomy
• ISSN: 1466-5034
• DOI: 10.1099/ijsem.0.006348

Two Gram-negative, aerobic, rod-shaped bacterial strains, 7MK25 and 6Y81 , were isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain 7MK25 showed the highest similarity (93.6 %) to AR4 , followed by DSM 9653 (93.3 %). Strain 6Y81 had the highest similarity of 97.9 % to RmlP026 , followed by RmlP001 (97.2 %). Phylogenomic analysis using the UBCG and PhyloPhlAn methods consistently showed that strain 7MK25 formed a sister clade to , while strain 6Y81 formed an independent clade within the genus , both in the order . The digital DNA-DNA hybridization and average nucleotide identity values between strains 7MK25 , 6Y81 and their close relatives were in the ranges of 19.1-29.9 % and 72.5-85.5 %, respectively. The major fatty acids of 7MK25 were summed feature 8 (C  ω7 /C  ω6 ), C cyclo ω8 , C and C cyclo, while those of 6Y81 were summed feature 8 (C  ω7 /C  ω6 ), C and C 3-OH. Strains 7MK25 and 6Y81 took diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as their dominant polar lipids, and Q-10 as their major respiratory quinone. On the basis of phenotypic and phylogenetic data, strain 7MK25 is proposed to represent a novel species of a novel genus with name gen. nov., sp. nov., within a novel family fam. nov., with 7MK25 (=KCTC 62738 =GDMCC 1.1452 ) as its type strain. Strain 6Y81 represents a novel species in the genus , for which the name sp. nov. (type strain 6Y81 =KACC 21 727 =GDMCC 1.2176 ) is proposed. fam. nov. with as the type genus is also proposed to solve the non-monophylectic problem of the family .