Terrirubrum flagellatum gen. nov., sp. nov. of Terrirubraceae fam. nov. and Lichenibacterium dinghuense sp. nov. from forest soil and proposal of Rhodoblastaceae fam. nov
Two Gram-negative, aerobic, rod-shaped bacterial strains, 7MK25 and 6Y81 , were isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain 7MK25 showed the highest similarity (93.6 %) to AR4 , followed by D...
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Published in | International journal of systematic and evolutionary microbiology Vol. 74; no. 4 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
01.04.2024
|
Subjects | |
Online Access | Get more information |
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Summary: | Two Gram-negative, aerobic, rod-shaped bacterial strains, 7MK25
and 6Y81
, were isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, PR China. Based on the results of 16S rRNA gene sequence analysis, strain 7MK25
showed the highest similarity (93.6 %) to
AR4
, followed by
DSM 9653
(93.3 %). Strain 6Y81
had the highest similarity of 97.9 % to
RmlP026
, followed by
RmlP001
(97.2 %). Phylogenomic analysis using the UBCG and PhyloPhlAn methods consistently showed that strain 7MK25
formed a sister clade to
, while strain 6Y81
formed an independent clade within the genus
, both in the order
. The digital DNA-DNA hybridization and average nucleotide identity values between strains 7MK25
, 6Y81
and their close relatives were in the ranges of 19.1-29.9 % and 72.5-85.5 %, respectively. The major fatty acids of 7MK25
were summed feature 8 (C
ω7
/C
ω6
), C
cyclo ω8
, C
and C
cyclo, while those of 6Y81
were summed feature 8 (C
ω7
/C
ω6
), C
and C
3-OH. Strains 7MK25
and 6Y81
took diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine as their dominant polar lipids, and Q-10 as their major respiratory quinone. On the basis of phenotypic and phylogenetic data, strain 7MK25
is proposed to represent a novel species of a novel genus with name
gen. nov., sp. nov., within a novel family
fam. nov., with 7MK25
(=KCTC 62738
=GDMCC 1.1452
) as its type strain. Strain 6Y81
represents a novel species in the genus
, for which the name
sp. nov. (type strain 6Y81
=KACC 21 727
=GDMCC 1.2176
) is proposed.
fam. nov. with
as the type genus is also proposed to solve the non-monophylectic problem of the family
. |
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ISSN: | 1466-5034 |
DOI: | 10.1099/ijsem.0.006348 |