In vitro electroporation of tobacco pollen

Tobacco pollen grains subjected in vitro to short electrical pulses of increasing intensities showed progressive decrease in germination and fluorescein diacetate staining. The ability of pollen to retain fluorescein was dependent on the field strength and the period of post pulse incubation. The de...

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Bibliographic Details
Published inPlant science (Limerick) Vol. 52; no. 1; pp. 135 - 139
Main Authors Mishra, K.P., Joshua, D.C., Bhatia, C.R.
Format Journal Article
LanguageEnglish
Published Shannon Elsevier Ireland Ltd 1987
Elsevier Science
Subjects
Biological and medical sciences
electroporation
fluorescence
Fundamental and applied biological sciences. Psychology
membrane permeability
Plant physiology and development
pollen germination
Tissue cultures, protoplasts
pollen germination
FDA
FCR
fluorescence
membrane permeability
electroporation
PFP
Electric field
Cell permeability
Dicotyledones
Germination
Angiospermae
Tissue culture
Spermatophyta
Pollen
Solanaceae
Nicotiana tabacum
In vitro
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Summary:Tobacco pollen grains subjected in vitro to short electrical pulses of increasing intensities showed progressive decrease in germination and fluorescein diacetate staining. The ability of pollen to retain fluorescein was dependent on the field strength and the period of post pulse incubation. The decrease in number of fluorescing pollen with the field treatment was due to increase in membrane permeability. The field induced leaks in the membrane are reversible and appear to possess discriminating properties towards solutes. Permeabilisation by pulsed field may enable incorporation of macromolecules in viable pollen.
ISSN:0168-9452
1873-2259
DOI:10.1016/0168-9452(87)90115-4