Phospholipase A2-like catalytic antibody

The phospholipase A2-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a kcat of 1.0 x 10(-2) min(-1) an...

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Bibliographic Details
Published inBioorganic & medicinal chemistry letters Vol. 9; no. 4; pp. 555 - 558
Main Authors ISOMURA, S, ITO, K, HARUNA, M
Format Journal Article
LanguageEnglish
Published Oxford Elsevier 22.02.1999
Subjects
Antibodies, Catalytic - metabolism
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Hydrolysis
Kinetics
Methods. Procedures. Technologies
Phospholipases A - metabolism
Phospholipases A2
Phospholipids - metabolism
Spectrometry, Mass, Fast Atom Bombardment
Substrate Specificity
Hydrolysis
Abzyme
Enantioselectivity
Regioselectivity
Lysophospholipid
Phosphatidylcholine
Phospholipid
Kinetics
Complex lipid
Lysophosphatidylcholine
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Summary:The phospholipase A2-like catalytic antibody 13C2-1F6 was elicited against the hapten 1 as the transition state analog for the hydrolysis of the C2 ester in the phospholipid. The Michaelis-Menten kinetics for the hydrolysis of the phospholipid 2 by 13C2-1F6 afforded a kcat of 1.0 x 10(-2) min(-1) and aKm of 71 microM. This antibody hydrolyzes the C2 ester in (R)-2, regio- and enantioselectively.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(99)00035-9