Alternative substrates and an inhibitor of the organophosphate acid anhydrase activities of the protozoan, Tetrahymena Thermophila

1. 4-Nitrophenyl ethyl(phenyl)phosphinate (NPEPP) is hydrolyzed by the opa anhydrases corresponding to the Tetrahymena-activities DFPase-1 and DFPase-2; and the Mazur-type opa anhydrase from hog kidney. 2. Mipafox was very slowly hydrolyzed by the partially purified extracts of the T. thermophila op...

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Published inComparative biochemistry and physiology. C, Comparative pharmacology Vol. 92; no. 2; pp. 211 - 216
Main Authors Landis, Wayne G., Chester, Nancy A., Haley, Mark V., Johnson, Dennis W., Tauber, Renee M., Durst, H.Dupont
Format Journal Article
LanguageEnglish
Published Oxford Elsevier B.V 1989
New York, NY Pergamon Press
Subjects
Biochemistry. Physiology. Immunology. Molecular biology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Protozoa
Protozoa
Tetrahymena thermophila
Enzymatic activity
Enzyme
Ciliata
Organophosphorus compounds
Inhibition
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Summary:1. 4-Nitrophenyl ethyl(phenyl)phosphinate (NPEPP) is hydrolyzed by the opa anhydrases corresponding to the Tetrahymena-activities DFPase-1 and DFPase-2; and the Mazur-type opa anhydrase from hog kidney. 2. Mipafox was very slowly hydrolyzed by the partially purified extracts of the T. thermophila opa anhydrases. 3. The DFP hydrolyzing activities, DFPase-1, DFPase-2, and DFPase-3, were strongly but reversibly inhibited by Mipafox. 4. In regard to the hydrolysis of NPEPP and the effect of Mipafox, the Tetrahymena opa anhydrases examined here more closely resemble the Mazur-type opa anhydrases.
ISSN:0306-4492
DOI:10.1016/0742-8413(89)90043-1