Genetic diversity analysis revealed the hot spot of Acmella paniculata(Wall ex DC.) R. K. Jansen existing in natural populations of Gujarat

• Published in: Genetic resources and crop evolution Vol. 69; no. 6; pp. 2249 - 2260
• Main Authors: Patel, Sveta, Purohit, Nikisha, Sapra, Palak, Solanki, Hitesh, Bishoyi, Ashok Kumar
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.08.2022; Springer Nature B.V
• Subjects: Acmella; Agriculture; Biomedical and Life Sciences; Conservation; Gene polymorphism; Genetic analysis; Genetic diversity; Genetic resources; Genotypes; Germplasm; Life Sciences; Markers; Natural populations; Notes on Neglected and Underutilized Crops; Plant Genetics and Genomics; Plant Physiology; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Polymorphism; Population genetics; Population studies; Populations; Resolution; Simple sequence repeats; Gujarat India; India; Genotype; Genetic diversity; ISSR marker; Biodiversity; Barcoding
• ISSN: 0925-9864; 1573-5109
• DOI: 10.1007/s10722-022-01400-y

Genetic diversity analysis contributes to the conservation, protection and utilization of genetic resources toward efficient management of germplasm. In this investigation, Inter Simple Sequence Repeats (ISSR) markers were used to analyze the genetic diversity of 32 genotypes of Acmella paniculata [(Wall. ex DC.) R. K. Jansen] collected from 12 different states of Gujarat, India. There were 113 loci amplified by 15 different ISSR markers with an average of 7.53 loci per primer. From the total 113 amplicons; 81 were polymorphic, 43 were monomorphic and 9 were found to be unique showing an average of 60.18% polymorphism. The primer (CT) 8 T produced a high value (0.65) of polymorphic information content; whereas, primer (TC) 8 RA produced a low value (0.21). The resolving power value of the primers ranged from 5.25 in primer (TC) 8 RA to 23.19 in primer (AC) 8 YT with an average of 12.17. The Jaccard’s similarity coefficients value ranged from 0.48 to 0.94. The highest percentage of similarity (94%) was found between accessions AP6 and AP16 which were collected from Panchmahal and Narmada respectively, as they are geographically closely located. Whereas, accessions AP2 from Banaskantha and AP7 from Panchmahal were most distantly related with the similarity value of 48%, as expected to their collection locations. The study revealed that there is no correlation between geographical distance and genetic diversity among the populations studied. The resultant data generated from ISSR molecular marker-based genetic diversity analysis of  A. paniculata  in Gujarat can provide a reference for the conservation and efficient management of the important Indian genetic resources.