A bioluminescent probe for imaging human cytochrome P450 2J2 activity in vitro and in vivo

• Published in: Sensors and actuators. B, Chemical Vol. 415; p. 135977
• Main Authors: Geng, Tingting, Ye, Ying, Hu, Liang, Jin, Yuyang, Zhu, Wujuan, Fang, Xiaoai, Hai, Zijuan, Shi, Xiang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.09.2024
• Subjects: Bioluminescence imaging; Human Cytochrome P450 2J2; Low detection limit; Tumor diagnosis; Ultrahigh signal-to-noise ratio; Tumor diagnosis; Bioluminescence imaging; Human Cytochrome P450 2J2; Ultrahigh signal-to-noise ratio; Low detection limit
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2024.135977

Human Cytochrome P450 2J2 (CYP 2J2) is closely associated with various physiological diseases. However, bioluminescence (BL) tools for monitoring CYP 2J2 activity have not yet been reported. Herein, we rationally designed a BL probe, PJ-Luc, capable of real-time imaging of CYP 2J2. In vitro studies demonstrated that PJ-Luc can effectively respond to exogenous CYP 2J2 with high selectivity and a low detection limit (LOD = 0.11 nM). Further, living cell experiments indicated that PJ-Luc can accurately quantify the ratio of endogenous CYP 2J2 amounts in HepG-2-Luc and A549-Luc cells. Moreover, an ultrahigh BL signal-to-noise ratio (S/N = 2.3×105) was achieved in tumor-bearing mouse models. These results suggest that PJ-Luc has great potential in the diagnosis of tumors and other CYP-2J2-related diseases. [Display omitted] •PJ-Luc was designed to exhibit high selectivity and an excellent signal-to-noise ratio specifically towards CYP 2J2.•PJ-Luc enabled an ultrahigh BL signal-to-noise ratio in vitro and in vivo.•PJ-Luc was suitable for distinguishing solid tumors with superior tumor-to-normal tissue ratios.