RAFT-synthesized POEGMA-b-P4VP block copolymers: preparation of nanosized micelles for anticancer drug release

• Published in: Polymer bulletin (Berlin, Germany) Vol. 79; no. 11; pp. 9575 - 9588
• Main Authors: Bayram, Nazende Nur, Topuzoğulları, Murat, İşoğlu, İsmail Alper, Dinçer İşoğlu, Sevil
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2022; Springer Nature B.V
• Subjects: Addition polymerization; Biocompatibility; Block copolymers; Breast cancer; Cancer therapies; Chain transfer; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chemotherapy; Complex Fluids and Microfluidics; Copolymerization; Dimethylformamide; Doxorubicin; Efficiency; Entrapment; Fourier transforms; Hemodialysis; Hydrophilic surfaces; Hydrophobicity; Light scattering; Luminous intensity; Micelles; NMR; Nuclear magnetic resonance; Organic Chemistry; Original Paper; Physical Chemistry; Polyethylene glycol; Polymer Sciences; Polymerization; Polymers; Scanning electron microscopy; Soft and Granular Matter; Spectrophotometry; Spectrum analysis; Synthesis; Toxicity; United States > US; Germany; Breast cancer; Micelle nanocarrier; RAFT; pH-responsive
• ISSN: 0170-0839; 1436-2449
• DOI: 10.1007/s00289-021-03964-8

To achieve high stability and biocompatibility in physiological environment, oligoethyleneglycol methacrylate (OEGMA) and 4-vinylpyridine (4VP)-based amphiphilic block copolymers were prepared as micellar carriers to deliver doxorubicin into tumor cells. First, macroinitiator of OEGMA was synthesized by RAFT polymerization at [M] 0 /[CTA] 0 /[I] 0 ratio of 100/1/0.2 in dimethylformamide (DMF) at 70 °C, in the presence of 4,4′-azobis(4-cyanovaleric acid) (ACVA) as initiator and 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTA) as chain transfer agent, respectively. It was followed by copolymerization with 4-VP at similar conditions. The formation of RAFT-mediated polymers was approved by FTIR, 1 H-NMR and GPC. For the preparation of drug-loaded micelles, a dialysis method was applied and hydrophobic doxorubicin, as a model drug, was entrapped into the micelles. Size distributions and morphologies of drug-loaded micelles were investigated by light scattering and scanning electron microscopy, respectively. Critical micelle concentration was estimated as 0.0019 mg/mL by measuring light scattering intensity in different polymer concentrations. Also, drug loading and entrapment efficiencies were calculated as 4.41% and 17.65% by measuring the DOX amount in the micelles, spectrophotometrically. At last, the drug-loaded micelles were applied to SKBR-3 breast cancer cell lines and revealed up to %40 cell inhibition at 48 and 72 h. As a result, these nanosized and biocompatible micelles can be used for the delivery of hydrophobic drugs, and they can also be modified for further targeting and imaging applications toward specific cancer cells. Graphical abstract